Template:SBB- Flocculation Assay

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'''Flocculation Assay Protocol'''
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To test cell-cell adhesion in the context of formation of flocculation (large clumps of boogers.)
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''Negative controls:''
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*DH10B (must be the same strain as the one you are testing)<br>
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plasmid 1363 (a plasmid that infers antibiotic resistance with no displayer. 1363 is strep binding peptide in the periplasm)
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''Positive Control''
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*DH10B (must be the same strain as the one you are testing)<br>
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with CPG-L2 displayer with a leucine zipper homo dimer part
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1.

Revision as of 12:58, 14 August 2009

Flocculation Assay Protocol

To test cell-cell adhesion in the context of formation of flocculation (large clumps of boogers.)

Negative controls:

  • DH10B (must be the same strain as the one you are testing)

plasmid 1363 (a plasmid that infers antibiotic resistance with no displayer. 1363 is strep binding peptide in the periplasm)

Positive Control

  • DH10B (must be the same strain as the one you are testing)

with CPG-L2 displayer with a leucine zipper homo dimer part

1.

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