Template:SBB10 ConstructionFiles BenB sbb01: Difference between revisions
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(New page: <pre> Construction of sbb01: N15 Protelomerase PCR BDBn15001F/BDBn15001R on Bca1455 (942 bp, gp=A) PCR BDBn15002F/BDBn15002R on Bca1485 (1020 bp, gp=B) ---------------------------------...) |
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Revision as of 13:35, 9 February 2010
Construction of sbb01: N15 Protelomerase PCR BDBn15001F/BDBn15001R on Bca1455 (942 bp, gp=A) PCR BDBn15002F/BDBn15002R on Bca1485 (1020 bp, gp=B) -------------------------------------------------------------- PCR BDBn15001F/BDBn15002R on A+B (1924 bp, EcoR1/BamH1) Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb01 {rbs.part!} BDBn15001F Forward retrieval, RBS introduction and BioBricking on Bca1455 CCATAgaattcATGagatctTCAATCTCGGAGGAACGGTAAGGAAGGAAAACatgAGCAAGGTAAAAATCGGTGAG BDBn15001R Reverse retrieval and SOE-site introduciton on Bca1455 CGCTTTTATCTTCACTGCGTTTTTTAGCTTGCCCTGAG BDBn15002F Forward retrieval and SOE-site introduciton on Bca1485 CTCAGGGCAAGCTAAAAAACGCAGTGAAGATAAAAGCG BDBn15002R Reverse retrieval and BioBricking on Bca1485 CTGATggatccGCTGTAGTACGTTTCCCATGCGG