Template:SBB10 ConstructionFiles Chel sbb02: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Michel Nofal (talk | contribs) (New page: sbb02: N15 Protelomerase PCR MNF11-F/MNF12-R on pBca9523-Bca (945 bp, gp = A) PCR MNF13-F/MNF14-R on pBca9523-Bca (1033 bp, gp = B) ------------- PCR MNF11-F/MNF14-R on A + B (1925 bp...) |
Michel Nofal (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
<pre> | |||
sbb02: N15 Protelomerase | sbb02: N15 Protelomerase | ||
PCR MNF11-F/MNF12-R on pBca9523-Bca (945 bp, gp = A) | PCR MNF11-F/MNF12-R on pBca9523-Bca (945 bp, gp = A) | ||
PCR MNF13-F/MNF14-R on pBca9523-Bca (1033 bp, gp = B) | PCR MNF13-F/MNF14-R on pBca9523-Bca (1033 bp, gp = B) | ||
------------- | ------------- | ||
PCR MNF11-F/MNF14-R on A + B (1925 bp, EcoRI/BamHI) | PCR MNF11-F/MNF14-R on A + B (1925 bp, EcoRI/BamHI) | ||
Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) | Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) | ||
Product is pBjk2741-sbb02 {<protel!} | Product is pBjk2741-sbb02 {<protel!} | ||
------------- | ------------- | ||
MNF11-F ccagtGAATTCgtccAGATCTAGCAAGGTAAAAATCGGTGAGTTG | MNF11-F ccagtGAATTCgtccAGATCTAGCAAGGTAAAAATCGGTGAGTTG | ||
MNF12-R ggttacgcttttatcttcactgcgTTTTTTAGCTTGCCCTGAGAAATTAACCG | MNF12-R ggttacgcttttatcttcactgcgTTTTTTAGCTTGCCCTGAGAAATTAACCG | ||
MNF13-F CGGTTAATTTCTCAGGGCAAGCTAAAAAAcgcagtgaagataaaagcgtaacc | MNF13-F CGGTTAATTTCTCAGGGCAAGCTAAAAAAcgcagtgaagataaaagcgtaacc | ||
MNF14-R gcagtggatccTCAGCTGTAGTACGTTTCCCATGC | MNF14-R gcagtggatccTCAGCTGTAGTACGTTTCCCATGC | ||
</pre> | |||
Revision as of 02:13, 9 February 2010
sbb02: N15 Protelomerase
PCR MNF11-F/MNF12-R on pBca9523-Bca (945 bp, gp = A)
PCR MNF13-F/MNF14-R on pBca9523-Bca (1033 bp, gp = B)
-------------
PCR MNF11-F/MNF14-R on A + B (1925 bp, EcoRI/BamHI)
Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb02 {<protel!}
-------------
MNF11-F ccagtGAATTCgtccAGATCTAGCAAGGTAAAAATCGGTGAGTTG
MNF12-R ggttacgcttttatcttcactgcgTTTTTTAGCTTGCCCTGAGAAATTAACCG
MNF13-F CGGTTAATTTCTCAGGGCAAGCTAAAAAAcgcagtgaagataaaagcgtaacc
MNF14-R gcagtggatccTCAGCTGTAGTACGTTTCCCATGC
