Template:SBB10 ConstructionFiles JennaK sbb28

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Biobricking of CA42 rep gene basic part sbb28

PCR repCA42-F and repCA42-R on pEC22-CA42 gene      (1496 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144                           (EcoRI/BamHI, 918+2472, L)
Product is pBca9523-repCA42			     {5'UTR.repCA42!} 
----------------------------
repCA42-F   Cloning of repCA42   ccaaaGAATTCtaaAGATCTcagctgaagtgaccggattag
repCA42-R   Cloning of repCA42   gctagGGATCCctattttccgcttttccagtttcg

JCA Notes

  • Correct except prefix should be ATG, not TAA
  • Size of vector dig slightly off
  • Reverse oligo can be shared with another part
  • Name of plasmid should be partName, not partDescription
 Biobricking of CA42 rep gene basic part sbb28

 PCR repCA42-F and KRM002 on pEC22-CA42 gene      (1496 bp, EcoRI/BamHI)
 Sub into pBca9523-Bca1144                           (EcoRI/BamHI, 910+2472, L)
 Product is pBca9523-sbb28     {5'UTR.repCA42!} 
 ----------------------------
 repCA42-F   Cloning of repCA42   
 ccaaaGAATTCatgAGATCTcagctgaagtgaccggattag
 KRM002   Reverse oligo for cloning of rbs.repCA42!    
 ctgatGGATCCctattttccgcttttccag
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