Template:SBB10 ConstructionFiles JoannaC sbb17: Difference between revisions
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Joanna Chen (talk | contribs) (New page: <pre> Construction of Zinc Finger Domain (zf+) (sbb17) Pool jc01 through jc16, assemble by PCA PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, ...) |
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jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC | jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC | ||
jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC | jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC | ||
jc05 PCA Assembly of sbb17 and sbb18 | jc05 PCA Assembly of sbb17 and sbb18 GTGAAAAACCGTTCGCTTGCGACATCTGCGGTCGTAAATTtGCa | ||
jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT | jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT | ||
jc07 PCA Assembly of sbb17 and sbb18 | jc07 PCA Assembly of sbb17 and sbb18 TCCGTACCCACACAGGTGAAAAACCGTTtGCaTGCGAtATCTGCGG | ||
jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT | jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT | ||
jc09 PCA Assembly of sbb17 CGTAACTTCTCTCGTTCTGACACCCTGTCTGAACACATCCGTACCCAC | jc09 PCA Assembly of sbb17 CGTAACTTCTCTCGTTCTGACACCCTGTCTGAACACATCCGTACCCAC | ||
jc10 PCA Assembly of sbb17 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGTTCAGAC | jc10 PCA Assembly of sbb17 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGTTCAGAC | ||
jc11 PCA Assembly of sbb17 | jc11 PCA Assembly of sbb17 GGTGTGGGTGGTACGGGTAGAACGAGCtGCaAATTTACGACCGCAG | ||
jc12 PCA Assembly of sbb17 TACCCGTACCACCCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC | jc12 PCA Assembly of sbb17 TACCCGTACCACCCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC | ||
jc13 PCA Assembly of sbb17 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACTCTCTGTCTAAACAC | jc13 PCA Assembly of sbb17 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACTCTCTGTCTAAACAC | ||
jc14 PCA Assembly of sbb17 GTTTTTCACCTGTGTGGGTACGGATGTGTTTAGACAGAGAGTCAGAACGA | jc14 PCA Assembly of sbb17 GTTTTTCACCTGTGTGGGTACGGATGTGTTTAGACAGAGAGTCAGAACGA | ||
jc15 PCA Assembly of sbb17 | jc15 PCA Assembly of sbb17 CTTTCAGGTTAGAACGCTGAGCGAAcTTACGtCCGCAGATaTCGCAtGCaA | ||
jc16 PCA Assembly of sbb17 TCGCTCAGCGTTCTAACCTGAAAGTTCACACCAAAATCCACCTGCGTGG | jc16 PCA Assembly of sbb17 TCGCTCAGCGTTCTAACCTGAAAGTTCACACCAAAATCCACCTGCGTGG | ||
</pre> | </pre> | ||
Revision as of 00:46, 10 February 2010
Construction of Zinc Finger Domain (zf+) (sbb17)
Pool jc01 through jc16, assemble by PCA
PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb17 {<zf+>}
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jc01 PCA Assembly of sbb17 and sbb18 AGTTCGAATTCATCAGATCTGCTCCGAAAAAAAAACGTAAAGTTGGTATCC
jc02 PCA Assembly of sbb17 and sbb18 CATAGCAGCCGGAACACCGTGGATACCAACTTTACGTTTTTTTTT
jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC
jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC
jc05 PCA Assembly of sbb17 and sbb18 GTGAAAAACCGTTCGCTTGCGACATCTGCGGTCGTAAATTtGCa
jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT
jc07 PCA Assembly of sbb17 and sbb18 TCCGTACCCACACAGGTGAAAAACCGTTtGCaTGCGAtATCTGCGG
jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT
jc09 PCA Assembly of sbb17 CGTAACTTCTCTCGTTCTGACACCCTGTCTGAACACATCCGTACCCAC
jc10 PCA Assembly of sbb17 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGTTCAGAC
jc11 PCA Assembly of sbb17 GGTGTGGGTGGTACGGGTAGAACGAGCtGCaAATTTACGACCGCAG
jc12 PCA Assembly of sbb17 TACCCGTACCACCCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC
jc13 PCA Assembly of sbb17 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACTCTCTGTCTAAACAC
jc14 PCA Assembly of sbb17 GTTTTTCACCTGTGTGGGTACGGATGTGTTTAGACAGAGAGTCAGAACGA
jc15 PCA Assembly of sbb17 CTTTCAGGTTAGAACGCTGAGCGAAcTTACGtCCGCAGATaTCGCAtGCaA
jc16 PCA Assembly of sbb17 TCGCTCAGCGTTCTAACCTGAAAGTTCACACCAAAATCCACCTGCGTGG
