Template:SBB10 ConstructionFiles JoannaC sbb17: Difference between revisions
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jc01 PCA Assembly of sbb17 and sbb18 | jc01 PCA Assembly of sbb17 and sbb18 AGTTCGAATTCATGAGATCTGCACCTAAGAAGAAACGCAAAGTGGGTATCC | ||
jc02 PCA Assembly of sbb17 and sbb18 | jc02 PCA Assembly of sbb17 and sbb18 GCCATTGCTGCTGGTACTCCATGGATACCCACTTTGCGTTTCTTCTT | ||
jc03 PCA Assembly of sbb17 and sbb18 | jc03 PCA Assembly of sbb17 and sbb18 TGGAGTACCAGCAGCAATGGCCGAACGTCCATTCCAGTGTCGTATTTGCA | ||
jc04 PCA Assembly of sbb17 and sbb18 | jc04 PCA Assembly of sbb17 and sbb18 TATCAGAGCGGGAGAAATTTCGCATGCAAATACGACACTGGAATGGAC | ||
jc05 PCA Assembly of sbb17 and sbb18 | jc05 PCA Assembly of sbb17 and sbb18 GCGAAAAGCCGTTCGCTTGCGACATTTGTGGACGTAAGTTTGCC | ||
jc06 PCA Assembly of sbb17 and sbb18 | jc06 PCA Assembly of sbb17 and sbb18 TCCGCATGCAGATCCGGCATTGGAAAGGTTTTTGACTACCAGTGT | ||
jc07 PCA Assembly of sbb17 and sbb18 | jc07 PCA Assembly of sbb17 and sbb18 TTCGCACTCACACAGGAGAAAAGCCTTTCGCGTGTGATATCTGCGGAC | ||
jc08 PCA Assembly of sbb17 and sbb18 | jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGGAGGTGAATTTTTGTGT | ||
jc09 PCA Assembly of sbb17 | jc09 PCA Assembly of sbb17 CGAAATTTCTCCCGCTCTGATACTCTGTCTGAACACATCCGAACGCAC | ||
jc10 PCA Assembly of sbb17 | jc10 PCA Assembly of sbb17 GCAAGCGAACGGCTTTTCGCCAGTGTGCGTTCGGATGTGTTCAGAC | ||
jc11 PCA Assembly of sbb17 | jc11 PCA Assembly of sbb17 GATCTTCGTATGAGTAGTCCGTGTACTTCGGGCGGCAAACTTACGTCCACAAATG | ||
jc12 PCA Assembly of sbb17 | jc12 PCA Assembly of sbb17 TACACGGACTACTCATACGAAGATCCACACTGGTAGTCAAAAACCTTTCCA | ||
jc13 PCA Assembly of sbb17 | jc13 PCA Assembly of sbb17 TGCCGGATCTGCATGCGGAATTTTAGCCGTAGTGACTCGCTCAGC | ||
jc14 PCA Assembly of sbb17 | jc14 PCA Assembly of sbb17 GCTTTTCTCCTGTGTGAGTGCGAATATGCTTGCTGAGCGAGTCACTACGG | ||
jc15 PCA Assembly of sbb17 | jc15 PCA Assembly of sbb17 CCTTGAGATTTGATCGCTGTGCGAACTTTCGTCCGCAGATATCACACGCGA | ||
jc16 PCA Assembly of sbb17 | jc16 PCA Assembly of sbb17 TCGCACAGCGATCAAATCTCAAGGTGCACACAAAAATTCACCTCCGTGGATC | ||
</pre> | </pre> | ||
Revision as of 03:02, 11 February 2010
Construction of Zinc Finger Domain (zf+) (sbb17)
Pool jc01 through jc16, assemble by PCA
PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb17 {<zf+>}
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jc01 PCA Assembly of sbb17 and sbb18 AGTTCGAATTCATGAGATCTGCACCTAAGAAGAAACGCAAAGTGGGTATCC
jc02 PCA Assembly of sbb17 and sbb18 GCCATTGCTGCTGGTACTCCATGGATACCCACTTTGCGTTTCTTCTT
jc03 PCA Assembly of sbb17 and sbb18 TGGAGTACCAGCAGCAATGGCCGAACGTCCATTCCAGTGTCGTATTTGCA
jc04 PCA Assembly of sbb17 and sbb18 TATCAGAGCGGGAGAAATTTCGCATGCAAATACGACACTGGAATGGAC
jc05 PCA Assembly of sbb17 and sbb18 GCGAAAAGCCGTTCGCTTGCGACATTTGTGGACGTAAGTTTGCC
jc06 PCA Assembly of sbb17 and sbb18 TCCGCATGCAGATCCGGCATTGGAAAGGTTTTTGACTACCAGTGT
jc07 PCA Assembly of sbb17 and sbb18 TTCGCACTCACACAGGAGAAAAGCCTTTCGCGTGTGATATCTGCGGAC
jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGGAGGTGAATTTTTGTGT
jc09 PCA Assembly of sbb17 CGAAATTTCTCCCGCTCTGATACTCTGTCTGAACACATCCGAACGCAC
jc10 PCA Assembly of sbb17 GCAAGCGAACGGCTTTTCGCCAGTGTGCGTTCGGATGTGTTCAGAC
jc11 PCA Assembly of sbb17 GATCTTCGTATGAGTAGTCCGTGTACTTCGGGCGGCAAACTTACGTCCACAAATG
jc12 PCA Assembly of sbb17 TACACGGACTACTCATACGAAGATCCACACTGGTAGTCAAAAACCTTTCCA
jc13 PCA Assembly of sbb17 TGCCGGATCTGCATGCGGAATTTTAGCCGTAGTGACTCGCTCAGC
jc14 PCA Assembly of sbb17 GCTTTTCTCCTGTGTGAGTGCGAATATGCTTGCTGAGCGAGTCACTACGG
jc15 PCA Assembly of sbb17 CCTTGAGATTTGATCGCTGTGCGAACTTTCGTCCGCAGATATCACACGCGA
jc16 PCA Assembly of sbb17 TCGCACAGCGATCAAATCTCAAGGTGCACACAAAAATTCACCTCCGTGGATC
