Template:SBB10 ConstructionFiles JoannaC sbb18: Difference between revisions
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Joanna Chen (talk | contribs) (New page: <pre> Construction of Zinc Finger Domain (zf-) (sbb18) Pool jc01 through jc08 and jc17 through jc24, assemble by PCA PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI) Sub into pBjk2741-...) |
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jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC | jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC | ||
jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC | jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC | ||
jc05 PCA Assembly of sbb17 and sbb18 | jc05 PCA Assembly of sbb17 and sbb18 GTGAAAAACCGTTCGCTTGCGACATCTGCGGTCGTAAATTtGCa | ||
jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT | jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT | ||
jc07 PCA Assembly of sbb17 and sbb18 | jc07 PCA Assembly of sbb17 and sbb18 TCCGTACCCACACAGGTGAAAAACCGTTtGCaTGCGAtATCTGCGG | ||
jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT | jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT | ||
jc17 PCA Assembly of sbb18 CGTAACTTCTCTCGTTCTGACAACCTGTCTGTTCACATCCGTACCCAC | jc17 PCA Assembly of sbb18 CGTAACTTCTCTCGTTCTGACAACCTGTCTGTTCACATCCGTACCCAC | ||
jc18 PCA Assembly of sbb18 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGAACAGAC | jc18 PCA Assembly of sbb18 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGAACAGAC | ||
jc19 PCA Assembly of sbb18 | jc19 PCA Assembly of sbb18 GATTTTGGTGTGGTTGATACGGTGAGCGTTACGtGCaAATTTACGACCGCAG | ||
jc20 PCA Assembly of sbb18 TCACCGTATCAACCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC | jc20 PCA Assembly of sbb18 TCACCGTATCAACCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC | ||
jc21 PCA Assembly of sbb18 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACACCCTGTCTGAAC | jc21 PCA Assembly of sbb18 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACACCCTGTCTGAAC | ||
jc22 PCA Assembly of sbb18 GTTTTTCACCTGTGTGGGTACGGATGTGTTCAGACAGGGTGTCAGAACGA | jc22 PCA Assembly of sbb18 GTTTTTCACCTGTGTGGGTACGGATGTGTTCAGACAGGGTGTCAGAACGA | ||
jc23 PCA Assembly of sbb18 | jc23 PCA Assembly of sbb18 CGGGTAGAACGAGCAGCGAAcTTACGtCCGCAGATaTCGCAtGCaA | ||
jc24 PCA Assembly of sbb18 TCGCTGCTCGTTCTACCCGTACCAACCACACCAAAATCCACCTGCGTGG | jc24 PCA Assembly of sbb18 TCGCTGCTCGTTCTACCCGTACCAACCACACCAAAATCCACCTGCGTGG | ||
</pre> | </pre> | ||
Revision as of 00:47, 10 February 2010
Construction of Zinc Finger Domain (zf-) (sbb18)
Pool jc01 through jc08 and jc17 through jc24, assemble by PCA
PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb18 {<zf->}
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jc01 PCA Assembly of sbb17 and sbb18 AGTTCGAATTCATCAGATCTGCTCCGAAAAAAAAACGTAAAGTTGGTATCC
jc02 PCA Assembly of sbb17 and sbb18 CATAGCAGCCGGAACACCGTGGATACCAACTTTACGTTTTTTTTT
jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC
jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC
jc05 PCA Assembly of sbb17 and sbb18 GTGAAAAACCGTTCGCTTGCGACATCTGCGGTCGTAAATTtGCa
jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT
jc07 PCA Assembly of sbb17 and sbb18 TCCGTACCCACACAGGTGAAAAACCGTTtGCaTGCGAtATCTGCGG
jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT
jc17 PCA Assembly of sbb18 CGTAACTTCTCTCGTTCTGACAACCTGTCTGTTCACATCCGTACCCAC
jc18 PCA Assembly of sbb18 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGAACAGAC
jc19 PCA Assembly of sbb18 GATTTTGGTGTGGTTGATACGGTGAGCGTTACGtGCaAATTTACGACCGCAG
jc20 PCA Assembly of sbb18 TCACCGTATCAACCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC
jc21 PCA Assembly of sbb18 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACACCCTGTCTGAAC
jc22 PCA Assembly of sbb18 GTTTTTCACCTGTGTGGGTACGGATGTGTTCAGACAGGGTGTCAGAACGA
jc23 PCA Assembly of sbb18 CGGGTAGAACGAGCAGCGAAcTTACGtCCGCAGATaTCGCAtGCaA
jc24 PCA Assembly of sbb18 TCGCTGCTCGTTCTACCCGTACCAACCACACCAAAATCCACCTGCGTGG
