Template:SBB10 ConstructionFiles KaiM sbb27

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sbb27: CA42 rep Protein

PCR KRM001 and KRM002 on pEC22-CA42  (987 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144            (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-sbb27         {rbs.repCA42!}
----------------------------
KRM001   Forward oligo for cloning of rbs.repCA42!   
ccataGAATTCatgAGATCTcccggagagaatcgtaacac
KRM002   Reverse oligo for cloning of rbs.repCA42!    
ctgatGGATCCctattttccgcttttccag

JCA Notes

  • correct
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