Template:SBB10 phiC31: Difference between revisions

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==This part is associated with phiC31 integration devices==
==This part is associated with phiC31 integration devices==
I wasn't sure which papers you would want to include. Here are the 4 that seemed most relevant, but you might want to cut one out:
phiC31 is a bacteriophage integrase.  It is somewhat similar in mechanism to Cre recombinase, but it acts on the asymmetric sites attB and attP.  It causes these sequences to be recombined to generate attL and attR sequences.  This system is useful for integrating large DNAs site-specifically into genomes.
 
- The first paper describes phiC31 integration in plastids
- The second paper describes Jin's proposed assay in one of its figures
- The last 2 papers address the same issue (adding a C-terminal) NLS to int increases efficiency)
- Jin may or may not have included one more paper at the top of his notes on this but the link was broken


'''You should read the following papers'''<br>
References: PMID 14996222, PMID 19002165, PMID 19439387, PMID 12034816
References: PMID 14996222, PMID 19002165, PMID 19439387, PMID 12034816
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Latest revision as of 23:50, 3 February 2010

This part is associated with phiC31 integration devices

phiC31 is a bacteriophage integrase. It is somewhat similar in mechanism to Cre recombinase, but it acts on the asymmetric sites attB and attP. It causes these sequences to be recombined to generate attL and attR sequences. This system is useful for integrating large DNAs site-specifically into genomes.

You should read the following papers
References: PMID 14996222, PMID 19002165, PMID 19439387, PMID 12034816