Template:SBB11Projectstdt1113

Averee Chang
To my Notebook

Welcome to your project page!

I've given you several parts to make.

• Design oligos to make your part
• Write up a proper construction file
• Enter your Features, Oligos, Parts, and Plasmids into Clotho

You should design your construction strategy to put your part into plasmid vectorName-Bca1144 (Where vectorName is indicated for each part) using EcoRI and BamHI. The sequences for all plasmids involved in our project are available on Clotho.

It is essential that you make your part in the correct vector, so make sure you're using the right one for each part!

Several references have been provided to give you some background on the biology of your part.

Also, use the link above left to upload a picture of yourself, your name, and anything else you'd like, and rename the link from "Your Name Here" to your name.

Finally, you should create a notebook on the main page of the wiki

 Pbad.VioABCDE.term  part jtk2914

This part encodes a Toxic Gene

This part contains a slightly toxic gene. All our parts encoding toxic genes ultimately need to be Pbad.rbs.gene composite parts in a pUC/ColE1 plasmid. Follow the instructions for figuring out how to make this composite part. In some cases, the part already exists and you'll just need to move it into the right vector. Other ones will require some oligos and PCR. Others still just involve making composite parts.

This part exists but requires an EcoRI/BamHI transfer

The VioABCDE set of genes encodes a biosynthetic pathway for producing violacein, a purple pigment. You'll notice when you work with this that your cells turn dark purple even if you don't put a promoter in front of it. It's very potent! (and also pretty toxic, we're going to figure out why) Your part was converted from a part made by the Cambridge 2009 iGEM team. More information on this pathway is available at their website (http://2009.igem.org/Team:Cambridge). This part already exists, but it is with the wrong vector. You'll need to do an Eco/Bam transfer to create the right plasmid. Your starting material is pBjh1601KC-jtk2914, and we need to subclone it into pBca9523-Bca1144#5.

 P_sfmC part sbb1126

This part encodes a stress promoter

You will be cloning this stress promoter from E. coli MG1655 genomic DNA. You will find that your Feature has already been entered into Clotho, and you should design a Part from this Feature and name it with whatever sbb11## is indicated next to the Feature. Note that some of these stress promoters contain internal restriction sites. You'll need to take care of those as you did in the tutorials. Since these are promoters, not coding sequences, there is no easy way of predicting whether the mutations you introduce will be neutral or not. So, just pick a single point mutation to introduce to get rid of the restriction site. The vector for your plasmid should be pBjh1601KC. You should transform your ligation into Lefty cells

 P_narP part sbb1109

This part encodes a stress promoter

You will be cloning this stress promoter from E. coli MG1655 genomic DNA. You will find that your Feature has already been entered into Clotho, and you should design a Part from this Feature and name it with whatever sbb11## is indicated next to the Feature. Note that some of these stress promoters contain internal restriction sites. You'll need to take care of those as you did in the tutorials. Since these are promoters, not coding sequences, there is no easy way of predicting whether the mutations you introduce will be neutral or not. So, just pick a single point mutation to introduce to get rid of the restriction site. The vector for your plasmid should be pBjh1601KC. You should transform your ligation into Lefty cells