Template:SBB11 stressPromoter: Difference between revisions
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<blockquote style="background: cornsilk; border: 1px solid rgb(153, 153, 153); padding: 1em;"> | <blockquote style="background: cornsilk; border: 1px solid rgb(153, 153, 153); padding: 1em;"> | ||
==This part encodes a stress promoter== | ==This part encodes a stress promoter== | ||
You will be cloning this stress promoter from ''E. coli'' MG1655 genomic DNA. You will find that your Feature has already been entered into Clotho, and you should design a Part from this Feature and name it with whatever sbb11## is indicated next to the Feature. Note that some of these stress promoters contain internal restriction sites. You'll need to take care of those as you did in the tutorials. Since these are promoters, not coding sequences, there is no easy way of predicting whether the mutations you introduce will be neutral or not. So, just pick a single point mutation to introduce to get rid of the restriction site. '''The vector for your plasmid should be pBjh1601KC.''' | You will be cloning this stress promoter from ''E. coli'' MG1655 genomic DNA. You will find that your Feature has already been entered into Clotho, and you should design a Part from this Feature and name it with whatever sbb11## is indicated next to the Feature. Note that some of these stress promoters contain internal restriction sites. You'll need to take care of those as you did in the tutorials. Since these are promoters, not coding sequences, there is no easy way of predicting whether the mutations you introduce will be neutral or not. So, just pick a single point mutation to introduce to get rid of the restriction site. '''The vector for your plasmid should be pBjh1601KC. You should transform your ligation into Lefty cells''' | ||
</blockquote> | </blockquote> | ||
__NOTOC__ | __NOTOC__ | ||
Latest revision as of 09:53, 23 February 2011
This part encodes a stress promoter
You will be cloning this stress promoter from E. coli MG1655 genomic DNA. You will find that your Feature has already been entered into Clotho, and you should design a Part from this Feature and name it with whatever sbb11## is indicated next to the Feature. Note that some of these stress promoters contain internal restriction sites. You'll need to take care of those as you did in the tutorials. Since these are promoters, not coding sequences, there is no easy way of predicting whether the mutations you introduce will be neutral or not. So, just pick a single point mutation to introduce to get rid of the restriction site. The vector for your plasmid should be pBjh1601KC. You should transform your ligation into Lefty cells
