Template:SBB12 homodimerizer: Difference between revisions
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==This part encodes a homodimer domain== | ==This part encodes a homodimer domain== | ||
You are going to put the homodimer on the C-terminus of a truncated ToxR. For this part, you're going to go off BioBricks. Kindov. Your final product will be a BioBrick, but you are going to use an ad hoc procedure to create it. You should examine [[Media:sbb2012_homodimerParts.pdf | this illustration]] which refers to two sequences: [[Media:pBca9525-Bca1834. | You are going to put the homodimer on the C-terminus of a truncated ToxR. For this part, you're going to go off BioBricks. Kindov. Your final product will be a BioBrick, but you are going to use an ad hoc procedure to create it. You should examine [[Media:sbb2012_homodimerParts.pdf | this illustration]] which refers to two sequences: [[Media:pBca9525-Bca1834.str]] and [[Media:pBca9525-Bca1839.str]]. The important thing here is that you think through the translation frame of the final product, and make sure that you add some linker between your sequence and the sequence 5' to it. You should also remove the start methionine from you homodimer, unless your project description explicitly says not to. | ||
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Revision as of 09:52, 5 February 2012
This part encodes a homodimer domain
You are going to put the homodimer on the C-terminus of a truncated ToxR. For this part, you're going to go off BioBricks. Kindov. Your final product will be a BioBrick, but you are going to use an ad hoc procedure to create it. You should examine this illustration which refers to two sequences: Media:pBca9525-Bca1834.str and Media:pBca9525-Bca1839.str. The important thing here is that you think through the translation frame of the final product, and make sure that you add some linker between your sequence and the sequence 5' to it. You should also remove the start methionine from you homodimer, unless your project description explicitly says not to.
