Template:SBB12 leucineZipper: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
JCAnderson (talk | contribs) (New page: <blockquote style="background: cornsilk; border: 1px solid rgb(153, 153, 153); padding: 1em;"> ==This part encodes a leucine zipper== We will be using a set of leucine zippers as homodimer...) |
(No difference)
|
Revision as of 11:14, 5 February 2012
This part encodes a leucine zipper
We will be using a set of leucine zippers as homodimer domains. In total, we'll have 8 of them all with different Kd's for homodimerization. However, the sequences only differ at the same 4 amino acid sites. This will allow us to test whether the activation of ToxR is dependent on the Kd of the homodimer that is attached to it. These sequences are entirely synthetic, but all should encode the following peptide:
VKELEDKNEELLS XX YH XX NEVARLKKLVGERGGC*
Where the x's are the amino acids I tell you. So, if I gave you the IILK zipper to make, the peptide you should encode is: VKELEDKNEELLSIIYHLKNEVARLKKLVGERGGC*
