Template:SSB-Protocols Col PCR: Difference between revisions
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Be sure to save some colonies on a block!<br> <br> | Be sure to save some colonies on a block!<br> <br> | ||
Do 4 colonies per transformation. <br> | |||
Pick colony and dab into PCR then dab into block. <br> | Pick colony and dab into PCR then dab into block. <br> | ||
Use 33-50ul of PCR mix per colony. <br> | Use 33-50ul of PCR mix per colony. <br> | ||
Revision as of 10:42, 8 June 2010
Be sure to save some colonies on a block!
Do 4 colonies per transformation.
Pick colony and dab into PCR then dab into block.
Use 33-50ul of PCR mix per colony.
Colony PCR Recipe:
| H2O | 41.5 | 41.5 |
| 10 mM dNTPs | 1 | 1 |
| 10x buffer (tube \"2\") | 5 | 5 |
| oligo 1 | 1 | 1 |
| oligo 2 | 1 | 1 |
| template | 1 | 1 |
| Taq polymerase \"1\" | 0.25 | 0.25 |
