Template:SSB-Protocols Col PCR
From OpenWetWare
Pick colony and dab into PCR. Be sure to save some colonies on a block!
Colony PCR Recipe:
H2O | 41.5 | 41.5 |
10 mM dNTPs | 1 | 1 |
10x buffer (tube \"2\") | 5 | 5 |
oligo 1 | 1 | 1 |
oligo 2 | 1 | 1 |
template | 1 | 1 |
Taq polymerase \"1\" | 0.25 | 0.25 |