Template:Undergrad Notebook 1: Difference between revisions

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==Description==
==Description==
* Add 1.5 uM BSA and 0.25 mM of HAuCl4 to 10 mL of 50mM acetate buffer (pH 5.46) at room temperature. The ratio of Au to BSA is 167 for optimum results.
<blockquote>Zein protein film formation with BSA conjugated NPs was carried out as follows. First of all, a clear solution of zein (10% w/v) in aqueous ethanol (90% v/v) along with glycerol (30% on zein weight basis) as plasticizer was prepared. Five grams of this filmogenic solution was placed in a 9 cm diameter plastic Petri dish and gently swirled to coat the bottom of the dish. It was placed without a lid on a level surface (checked with a spirit level) in an oven at 40°C for 24 h.</blockquote>
** Note: distilled water used to rinse gold and BSA into mixture from weigh boats.
** Four-place balance used to determine weights. Actual weights determined to be g BSA and g gold.
* Heat mixture at 80°C for four hours (actual temperature varies closer to 70°C).
* Using a UV-Vis Spectrophotometer, obtain spectra every 30 minutes, starting at t=0 minutes
 
 
*The solution is covered in aluminum foil and stored at room temperature overnight.


==Data==
==Data==

Revision as of 17:28, 6 September 2011

Usage

This template can be used for Entry Pages for the notebooks of students in an undergraduate lab course.

Upon creating a new notebook, customize the Entry Page by placing the tag for this template between the header and footer, as follows:

<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
{{subst:Undergrad Notebook 1}}
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Afterwards, each new page added for a notebook will have four sections:

  • Objective
  • Description (ie, experimental)
  • Data
  • Notes

It will also have category tags for the course and experiment, which should be provided to the students by the instructor.