Template:Undergrad Notebook 1: Difference between revisions

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==Objective==
==Objective==
*Add your objective here
stuff [[User:Matt Hartings|Matt Hartings]] 21:41, 6 September 2011 (EDT)


==Description==
==Description==
* Add 0.0015 mM BSA and 0.25 mM of HAuCl4 to 10 mL of 50mM acetate buffer (pH 5.46) at room temperature. The ratio of Au to BSA is 167 for optimum results.
*Add description here
** Note: distilled water used to rinse gold and BSA into mixture from weigh boats.
more stuff [[User:Matt Hartings|Matt Hartings]] 21:43, 6 September 2011 (EDT)
** Four-place balance used to determine weights. Actual weights determined to be 0.0011g BSA and 0.0004g gold.
* Heat mixture at 80°C for four hours (actual temperature varies closer to 70°C).
* Using a UV-Vis Spectrophotometer, obtain spectra every 30 minutes, starting at t=0 minutes
* Calculations:
::'''''Au'''''
::0.25mM Au = 0.00025 mol / L = 2.5 x 10<sup>-7</sup> mol / mL
 
::2.5 x 10<sup>-7</sup> mol / mL = x / 10 mL
 
::x = 2.5 x 10<sup>-6</sup> mol / mL x 196.967 g/mol = 4.92 x 10<sup>-4</sup>g
 
::'''''BSA'''''
::0.0015mM BSA = 0.0000015 mol / L = 1.5 x 10<sup>-9</sup> mol / mL
 
::1.5 x 10<sup>-9</sup> mol / mL = x / 10 mL
 
::x = 1.5 x 10<sup>-9</sup> mol / mL x 66776 g/mol = 1.00 x 10<sup>-3</sup>g
 
*The solution is covered in aluminum foil and stored at room temperature overnight.


==Data==
==Data==

Latest revision as of 18:43, 6 September 2011

Usage

This template can be used for Entry Pages for the notebooks of students in an undergraduate lab course.

Upon creating a new notebook, customize the Entry Page by placing the tag for this template between the header and footer, as follows:

<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
{{subst:Undergrad Notebook 1}}
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Afterwards, each new page added for a notebook will have four sections:

  • Objective
  • Description (ie, experimental)
  • Data
  • Notes

It will also have category tags for the course and experiment, which should be provided to the students by the instructor.



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