Terminal Transferase

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (10:24, 24 May 2007) (view source)
 
(One intermediate revision not shown.)
Line 1: Line 1:
 +
{{stub}}
 +
 +
Terminal Transferase (TdT) adds any deoxynucleotides in a template-independent manner to the 3' end of DNA.
Terminal Transferase (TdT) adds any deoxynucleotides in a template-independent manner to the 3' end of DNA.
Line 7: Line 10:
==Links==
==Links==
http://www.neb.com/nebecomm/products/productM0252.asp
http://www.neb.com/nebecomm/products/productM0252.asp
 +
 +
[[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:DNA]]

Current revision


Terminal Transferase (TdT) adds any deoxynucleotides in a template-independent manner to the 3' end of DNA.

People usually use this to poly-tail with a certain nucleotide. According to NEB's site, with an excess of a single nucleotide, the tail lengths with TdT after 15 minutes are dA: 300-500, dC: 100-150, dG: 15-25, dT: 250-350

However, here's some anecdotal evidence I have of nucleotide distribution: I used TdT for 30 minutes @37C in NEB4+CoCl2, with all 4 nucleotides (0.25mM each). Of 4 colonies sequenced, the random sequences were of lengths: 133, 198, 42, 69. The stats of the sequence generated was: 132 A, 54 T, 81 C, 175 G (total 442 nt). This shows a heavy G-bias which doesn't seem to agree with NEB. --Austin 19:35, 13 March 2006 (EST)

Links

http://www.neb.com/nebecomm/products/productM0252.asp

Personal tools