Terrific Broth: Difference between revisions
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(Rephrasing of potassium phosphate solution for better clarity) |
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#24 g Bacto yeast extract | #24 g Bacto yeast extract | ||
#4 mL Glycerol | #4 mL Glycerol | ||
#100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, prepared separately | #100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution. | ||
==Protocol== | ==Protocol== | ||
Revision as of 08:15, 19 December 2006
Summary
Terrific broth (TB, not to be confused with T Broth)
Ingredients
- 12 g Bacto tryptone
- 24 g Bacto yeast extract
- 4 mL Glycerol
- 100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution.
Protocol
- Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL
- Pour into 2 L flask (or greater)
- Autoclave (liquid cycle)
- Allow liquid to cool to less than 60 degrees Centrigrade
- Add 100 mL potassium phosphate solution
