Tetracycline - Revision history

Oliver Wright: Advised on solvent for tetracycline stock

Oliver Wright — Wed, 13 Feb 2013 14:31:51 GMT

Advised on solvent for tetracycline stock

←Older revision		Revision as of 14:31, 13 February 2013
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	*Using a plasmid with copy number 15-20, 20μg/ml tet has proved a successful selector. The reduction in growth rate, described above was significant.		*Using a plasmid with copy number 15-20, 20μg/ml tet has proved a successful selector. The reduction in growth rate, described above was significant.
	*The RajBhandary lab uses 5μg/ml and see a minimal reduction in growth rate and never lose plasmid from their cells.		*The RajBhandary lab uses 5μg/ml and see a minimal reduction in growth rate and never lose plasmid from their cells.
-	*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.	+	*Stock solution is 15 mg/ml in 50% ethanol (note: using 95-100% ethanol is much better for dissolving stock powder). Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.
	*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media		*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media

Maximilian Peters: /* Properties */ units added

Maximilian Peters — Mon, 19 Jan 2009 14:37:40 GMT

Properties: units added

←Older revision		Revision as of 14:37, 19 January 2009
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	==Properties==		==Properties==
-	*'''Molecular Weight''' - 480.9g (Hydrochloride salt), 444.4 (free base)	+	*'''Molecular Weight''' - 480.9 g/mol (Hydrochloride salt), 444.4 g/mol (free base)

	==References==		==References==

Maximilian Peters: /* Properties */ molecular weight clarified

Maximilian Peters — Mon, 19 Jan 2009 14:35:35 GMT

Properties: molecular weight clarified

←Older revision		Revision as of 14:35, 19 January 2009
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	==Properties==		==Properties==
-	*'''Molecular Weight''' - 480.9g	+	*'''Molecular Weight''' - 480.9g (Hydrochloride salt), 444.4 (free base)
-	** (~~This value doesn't agree with the number given in the Wikipedia link below~~)	+

	==References==		==References==

Reshma P. Shetty at 17:48, 26 July 2007

Reshma P. Shetty — Thu, 26 Jul 2007 17:48:54 GMT

←Older revision		Revision as of 17:48, 26 July 2007
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	*[[Molecular Cloning]]		*[[Molecular Cloning]]
	*[[Wikipedia:Tetracycline\|Wikipedia Tetracycline page]]		*[[Wikipedia:Tetracycline\|Wikipedia Tetracycline page]]
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Austin J. Che at 19:26, 27 April 2007

Austin J. Che — Fri, 27 Apr 2007 19:26:42 GMT

←Older revision		Revision as of 19:26, 27 April 2007
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	*Fusaric acid can be used to select against tetracycline resistant colonies. See PMID 6259126, and modifications PMID 7007341.		*Fusaric acid can be used to select against tetracycline resistant colonies. See PMID 6259126, and modifications PMID 7007341.
	*Nickel ions can also be used for tetR counterselection. See PMID 8954882.		*Nickel ions can also be used for tetR counterselection. See PMID 8954882.
-
-	~~==Plate Color Code==~~
-	~~Yellow stripe~~

	==Properties==		==Properties==

Tk at 03:02, 7 January 2006

Tk — Sat, 07 Jan 2006 03:02:02 GMT

←Older revision		Revision as of 03:02, 7 January 2006
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	==Resistance Mechanism==		==Resistance Mechanism==
-	There are a number of resistance mechanisms, most require tetracycline antiporters. The most commonly used is the tetA(C) gene. The gene product of tetA(C) forms a pore in the inner membrane and exports one tet molecule for every proton imported. [[Molecular Cloning]] states that tet resistance has a number of adverse affects on the cell, chief among those being a reduction in growth rate and viability.	+	There are a number of resistance mechanisms, most require tetracycline antiporters. The most commonly used is the tetA(C) gene. The gene product of tetA(C) forms a pore in the inner membrane and exports one tet molecule for every proton imported. [[Molecular Cloning]] states that tet resistance has a number of adverse affects on the cell, chief among those being a reduction in growth rate and viability.

	==Working concentration and Stock Solutions==		==Working concentration and Stock Solutions==
Line 10:		Line 10:
	*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.		*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.
	*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media		*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media
		+
		+	==Counterselection techniques==
	*Fusaric acid can be used to select against tetracycline resistant colonies. See PMID 6259126, and modifications PMID 7007341.		*Fusaric acid can be used to select against tetracycline resistant colonies. See PMID 6259126, and modifications PMID 7007341.
	*Nickel ions can also be used for tetR counterselection. See PMID 8954882.		*Nickel ions can also be used for tetR counterselection. See PMID 8954882.

Tk: /* Working concentration and Stock Solutions */

Tk — Sat, 07 Jan 2006 03:00:33 GMT

Working concentration and Stock Solutions

←Older revision		Revision as of 03:00, 7 January 2006
Line 10:		Line 10:
	*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.		*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.
	*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media		*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media
		+	*Fusaric acid can be used to select against tetracycline resistant colonies. See PMID 6259126, and modifications PMID 7007341.
		+	*Nickel ions can also be used for tetR counterselection. See PMID 8954882.

	==Plate Color Code==		==Plate Color Code==

Tk: /* Working concentration and Stock Solutions */

Tk — Fri, 06 Jan 2006 22:51:55 GMT

Working concentration and Stock Solutions

←Older revision		Revision as of 22:51, 6 January 2006
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	*The RajBhandary lab uses 5μg/ml and see a minimal reduction in growth rate and never lose plasmid from their cells.		*The RajBhandary lab uses 5μg/ml and see a minimal reduction in growth rate and never lose plasmid from their cells.
	*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.		*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.
		+	*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media

	==Plate Color Code==		==Plate Color Code==

Austin J. Che at 19:57, 19 December 2005

Austin J. Che — Mon, 19 Dec 2005 19:57:50 GMT

←Older revision		Revision as of 19:57, 19 December 2005
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	==Properties==		==Properties==
	*'''Molecular Weight''' - 480.9g		*'''Molecular Weight''' - 480.9g
		+	** (This value doesn't agree with the number given in the Wikipedia link below)

	==References==		==References==
	*[[Molecular Cloning]]		*[[Molecular Cloning]]
	*[[Wikipedia:Tetracycline\|Wikipedia Tetracycline page]]		*[[Wikipedia:Tetracycline\|Wikipedia Tetracycline page]]

Tk: /* Working concentration and Stock Solutions */

Tk — Mon, 19 Dec 2005 19:38:09 GMT

Working concentration and Stock Solutions

←Older revision		Revision as of 19:38, 19 December 2005
Line 8:		Line 8:
	*Using a plasmid with copy number 15-20, 20μg/ml tet has proved a successful selector. The reduction in growth rate, described above was significant.		*Using a plasmid with copy number 15-20, 20μg/ml tet has proved a successful selector. The reduction in growth rate, described above was significant.
	*The RajBhandary lab uses 5μg/ml and see a minimal reduction in growth rate and never lose plasmid from their cells.		*The RajBhandary lab uses 5μg/ml and see a minimal reduction in growth rate and never lose plasmid from their cells.
-	*Stock solution is 5 mg/ml in ethanol. ~~Higher concentrations are not soluble at -20 C~~. Solutions are light sensitive.	+	*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive.

	==Plate Color Code==		==Plate Color Code==