The BioBricks Foundation:Standards/Technical/Measurement

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(Promoter measurement test)
(Promoter measurement test)
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=Promoter measurement test=
=Promoter measurement test=
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[[Image:Promoter tester demo.jpg|500px|thumb|left|Plate reader measurements of the promoter set]]
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[[Image:Promoter tester demo.jpg|500px|thumb|left|Plate reader & FACS measurements of the promoter set at MIT]]
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[[Image:Mfa0910100 BBF FACS.jpg|500px|thumb|left|FACS of promoter set at Lawrence Berkeley]]
[[Image:Mfa0910100 BBF FACS.jpg|500px|thumb|left|FACS of promoter set at Lawrence Berkeley]]
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==Notes==
==Notes==
*[[Notebook:Jason R. Kelly/Projects/BBF Meas Standards]] - Jason's lab notebook.
*[[Notebook:Jason R. Kelly/Projects/BBF Meas Standards]] - Jason's lab notebook.
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*[[Image:Measkit promoter FACS.PNG|500px|thumb|left|Histogram of the FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).]]
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*[[Image:Measkit promoter FACS.PNG|500px|thumb|left|Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).]]

Revision as of 07:00, 4 March 2008

Contents

Briefing on Measuring Promoters & RBSs

Video from Jason Kelly introducing a standard measurement kit for characterizing BioBrick promoters and RBSs.

Promoter measurement test

Plate reader & FACS measurements of the promoter set at MIT
Plate reader & FACS measurements of the promoter set at MIT


FACS of promoter set at Lawrence Berkeley
FACS of promoter set at Lawrence Berkeley


Protocols

Notes

  • Notebook:Jason R. Kelly/Projects/BBF Meas Standards - Jason's lab notebook.
  • Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).
    Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).
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