The BioBricks Foundation:Standards/Technical/Measurement

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==Notes==
==Notes==
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*[[Notebook:Jason R. Kelly/Projects/BBF Meas Standards]] - Jason's lab notebook.
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*[[Image:Measkit promoter FACS.PNG|500px|thumb|left|Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).]]
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[[Notebook:Jason R. Kelly/Projects/BBF Meas Standards]] - Jason's lab notebook.
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[[Image:Measkit promoter FACS.PNG|500px|thumb|left|Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).]]
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=Resources about measuring gene expression =
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<biblio>
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#1 pmid=10860516
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</biblio>

Revision as of 05:26, 6 March 2008

Contents

Briefing on Measuring Promoters & RBSs

Video from Jason Kelly introducing a standard measurement kit for characterizing BioBrick promoters and RBSs.

Promoter measurement test

Plate reader & FACS measurements of the promoter set at MIT
Plate reader & FACS measurements of the promoter set at MIT


FACS of promoter set at Lawrence Berkeley
FACS of promoter set at Lawrence Berkeley


Protocols

Notes

Notebook:Jason R. Kelly/Projects/BBF Meas Standards - Jason's lab notebook.

Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).
Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).

Resources about measuring gene expression

  1. Dyer BW, Ferrer FA, Klinedinst DK, and Rodriguez R. . pmid:10860516. PubMed HubMed [1]
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