The BioBricks Foundation:Standards/Technical/Measurement

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(Briefing on Measuring Promoters & RBSs)
(Briefing on Measuring Promoters & RBSs)
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*[http://video.google.com/googleplayer.swf?docId=3783852973289336417&hl=en Video] from Jason Kelly introducing a standard measurement kit for characterizing BioBrick promoters and RBSs.
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*[http://video.google.com/googleplayer.swf?docId=3783852973289336417&hl=en Video] from [[User:Jason R. Kelly|Jason Kelly]] introducing a standard measurement kit for characterizing BioBrick promoters and RBSs.
=Promoter measurement test=
=Promoter measurement test=

Revision as of 14:25, 13 March 2008

Contents

Goals of the BBF Measurement Working Group

  • We aim to submit the following standards for comment in advance of the June workshop:
    • the definition of a consensus relative fluorescence unit of measure - a 'Biobricks E. coli GFP fluorescence unit'
    • a standard method for measuring promoter strengths

Briefing on Measuring Promoters & RBSs

  • Video from Jason Kelly introducing a standard measurement kit for characterizing BioBrick promoters and RBSs.

Promoter measurement test

Plate reader & FACS measurements of the promoter set at MIT
Plate reader & FACS measurements of the promoter set at MIT


FACS of promoter set at Lawrence Berkeley
FACS of promoter set at Lawrence Berkeley


Protocols

Notes

Notebook:Jason R. Kelly/Projects/BBF Meas Standards - Jason's lab notebook.

Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).
Histogram of the MIT FACS data above, sorry there's no legend, but just wanted to show that the peaks aren't multimodal. The numbers reported as 'FACS' in the bar chart are the geometric means of these peaks reported relative to I20260 (dark blue).

Resources about measuring gene expression

  1. Dyer BW, Ferrer FA, Klinedinst DK, and Rodriguez R. . pmid:10860516. PubMed HubMed [1]
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