The BioBricks Foundation:Standards/Technical/Measurement: Difference between revisions
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[[Image:Mfa0910100 BBF FACS.jpg|500px|thumb|left|FACS of promoter set at Lawrence Berkeley]] | [[Image:Mfa0910100 BBF FACS.jpg|500px|thumb|left|FACS of promoter set at Lawrence Berkeley]] | ||
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[[Image:DDM_promoter_chart.jpg|500px|thumb|left|FACS of promoter set at | [[Image:DDM_promoter_chart.jpg|500px|thumb|left|FACS measurements of promoter set at Johns Hopkins]] | ||
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==Notebooks== | ==Notebooks== | ||
*[[Notebook:Jason R. Kelly/Projects/BBF Meas Standards]] - Jason's lab notebook. | *[[Notebook:Jason R. Kelly/Projects/BBF Meas Standards]] - Jason's lab notebook. | ||
*[[User:Dileep_D._Monie/Notebook]] - Dileep's lab notebook | *[[User:Dileep_D._Monie/Notebook/BBF_Standards_Measurement]] - Dileep's lab notebook | ||
==Notes== | ==Notes== | ||
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</biblio> | </biblio> | ||
*[http://www.jstor.org/ | *[http://www.jstor.org/stable/301998 The Ohm is where the art is (Bruce Hunt)] - amazing history of standard setting in electrical engineering | ||
*[http://books.google.com/books?id=8M0LAAAAYAAJ Reports of the Committee on Electrical Standards (1873)] - The committee was responsible for determining "what would be the most convenient unit of resistance, and second what would be the best form and material for the standard representing that unit". | |||
Latest revision as of 15:55, 3 November 2008
Goals of the BBF Measurement Working Group
- We aim to submit the following standards for comment in advance of the June workshop:
- the definition of a consensus relative fluorescence unit of measure - a 'Biobricks E. coli GFP fluorescence unit'
- a standard method for measuring promoter strengths
Briefing on Measuring Promoters & RBSs
<html> <embed style="width:400px; height:326px;" id="VideoPlayback" type="application/x-shockwave-flash" src="http://video.google.com/googleplayer.swf?docId=3783852973289336417&hl=en" flashvars=""> </embed> </html>
- Video from Jason Kelly introducing a standard measurement kit for characterizing BioBrick promoters and RBSs. (feel free to comment via talk page, blog, email, smoke signal, etc.)
Promoter measurement test
Protocols
Notebooks
- Notebook:Jason R. Kelly/Projects/BBF Meas Standards - Jason's lab notebook.
- User:Dileep_D._Monie/Notebook/BBF_Standards_Measurement - Dileep's lab notebook
Notes
 |
References
- Dyer BW, Ferrer FA, Klinedinst DK, and Rodriguez R. A noncommercial dual luciferase enzyme assay system for reporter gene analysis. Anal Biochem. 2000 Jun 15;282(1):158-61. DOI:10.1006/abio.2000.4605 |
- The Ohm is where the art is (Bruce Hunt) - amazing history of standard setting in electrical engineering
- Reports of the Committee on Electrical Standards (1873) - The committee was responsible for determining "what would be the most convenient unit of resistance, and second what would be the best form and material for the standard representing that unit".
