The mRNA Decay Resource:Protocols and Resources

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(New page: {{mRNA Decay Top}} ===Protocols=== ==Yeast protocols from the Atkin lab== <DD><A HREF="RNAextrc.pdf" TARGET="_blank">Yeast RNA extraction</A> <DL> <DD>Support Protocol: <A HREF=...)
(Yeast protocols from the Atkin lab)
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===Protocols===
===Protocols===
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==Yeast protocols from the Atkin lab==
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==Yeast protocols==
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<DD><A HREF="RNAextrc.pdf" TARGET="_blank">Yeast RNA extraction</A>
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    <DL>
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      <DD>Support Protocol: <A HREF="rnapheno.pdf" TARGET="_blank">Preparation
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      of buffer saturated phenol</A>
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    </DL>
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From the Atkin lab <br>
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    <DD><A HREF="mrnahalf.pdf">Measurement of mRNA half lives</A>
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[Media:RNAextrc.pdf Total RNA extraction] <br>
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    <DD><A HREF="PlyriRNA.pdf">Yeast polyribosome preparation - RNA</A>
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    Support Protocol: [Media:rnapheno.pdf Preparation of buffer saturated phenol] <br>
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    <DD><A HREF="RNAgels.pdf">Electrophoresis of RNA through gels containing
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[Media:mrnahalf.pdf Measurement of mRNA half lives] <br>
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    formaldehyde</A>
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[Media:PlyriRNA.pdf Yeast polyribosome preparation for RNA] <br>
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    <DD><A HREF="NorthRNA.pdf">Northern transfer of RNA</A>
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[Media:RNAgels.pdf Electrophoresis of RNA through gels containing formaldehyde] <br>
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    <DD><A HREF="dnaprobe.pdf">Preparation of oligolabeled probes</A>
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[Media:NorthRNA.pdf Northern transfer of RNA] <br>
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[Media:dnaprobe.pdf Preparation of oligolabeled probes] <br>
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    <DD><A HREF="hybrdRNA.pdf">Hybridization of RNA blots</A>
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[Media:hybrdRNA.pdf Hybridization of RNA blots] <br>
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    <DD><A HREF="Remov32P.pdf">Removal of oligolabeled probes for GeneScreen
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[Media:Remov32P.pdf Removal of oligolabeled probes for GeneScreen <I>Plus</I> membranes] <br>
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    <I>Plus</I> membranes</A>
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[Media:plyriprot.pdf Yeast polyribosome preparation - Proteins] <br>
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    <DD><A HREF="plyriprot.pdf">Yeast polyribosome preparation - Proteins</A>
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[Media:protgel.pdf Gel electrophoresis of proteins in denaturing conditions] <br>
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    <DD><A HREF="protgel.pdf">Gel electrophoresis of proteins in denaturing
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[Media:protran.pdf Electrotransfer of proteins from polyacrylamide gels to membranes] <br>
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    conditions</A>
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[Media:imblot.pdf Immunoblotting] <br>
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    <DD><A HREF="protran.pdf">Electrotransfer of proteins from polyacrylamide
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    gels to membranes</A>
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    <DD><A HREF="imblot.pdf">Immunoblotting</A>
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Revision as of 12:59, 17 June 2008

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Protocols

Yeast protocols

From the Atkin lab
[Media:RNAextrc.pdf Total RNA extraction]

    Support Protocol: [Media:rnapheno.pdf Preparation of buffer saturated phenol] 

[Media:mrnahalf.pdf Measurement of mRNA half lives]
[Media:PlyriRNA.pdf Yeast polyribosome preparation for RNA]
[Media:RNAgels.pdf Electrophoresis of RNA through gels containing formaldehyde]
[Media:NorthRNA.pdf Northern transfer of RNA]
[Media:dnaprobe.pdf Preparation of oligolabeled probes]
[Media:hybrdRNA.pdf Hybridization of RNA blots]
[Media:Remov32P.pdf Removal of oligolabeled probes for GeneScreen Plus membranes]
[Media:plyriprot.pdf Yeast polyribosome preparation - Proteins]
[Media:protgel.pdf Gel electrophoresis of proteins in denaturing conditions]
[Media:protran.pdf Electrotransfer of proteins from polyacrylamide gels to membranes]
[Media:imblot.pdf Immunoblotting]

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