The mRNA Decay Resource:Protocols and Resources

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==Yeast protocols==
==Yeast protocols==
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From the Atkin lab <br>
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=From the Atkin lab= <br>
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[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [Media:rnapheno.pd| Preparation of buffer saturated phenol] <br>
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[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [Media:rnapheno.pdf|Preparation of buffer saturated phenol] <br>
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[Media:mrnahalf.pdf Measurement of mRNA half lives] <br>
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[Media:mrnahalf.pdf|Measurement of mRNA half lives] <br>
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[Media:PlyriRNA.pdf Yeast polyribosome preparation for RNA] <br>
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[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA] <br>
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[Media:RNAgels.pdf Electrophoresis of RNA through gels containing formaldehyde] <br>
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[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde] <br>
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[Media:NorthRNA.pdf Northern transfer of RNA] <br>
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[Media:NorthRNA.pdf|Northern transfer of RNA] <br>
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[Media:dnaprobe.pdf Preparation of oligolabeled probes] <br>
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[Media:dnaprobe.pdf|Preparation of oligolabeled probes] <br>
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[Media:hybrdRNA.pdf Hybridization of RNA blots] <br>
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[Media:hybrdRNA.pdf|Hybridization of RNA blots] <br>
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[Media:Remov32P.pdf Removal of oligolabeled probes for GeneScreen <I>Plus</I> membranes] <br>
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[Media:Remov32P.pdf|Removal of oligolabeled probes for GeneScreen <I>Plus</I> membranes] <br>
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[Media:plyriprot.pdf Yeast polyribosome preparation - Proteins] <br>
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[Media:plyriprot.pdf|Yeast polyribosome preparation - Proteins] <br>
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[Media:protgel.pdf Gel electrophoresis of proteins in denaturing conditions] <br>
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[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions] <br>
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[Media:protran.pdf Electrotransfer of proteins from polyacrylamide gels to membranes] <br>
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[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes] <br>
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[Media:imblot.pdf Immunoblotting] <br>
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[Media:imblot.pdf|Immunoblotting] <br>

Revision as of 14:45, 17 June 2008

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Protocols

Yeast protocols

=From the Atkin lab=
Total RNA extraction Support Protocol: [Media:rnapheno.pdf|Preparation of buffer saturated phenol]
[Media:mrnahalf.pdf|Measurement of mRNA half lives]
[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]
[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]
[Media:NorthRNA.pdf|Northern transfer of RNA]
[Media:dnaprobe.pdf|Preparation of oligolabeled probes]
[Media:hybrdRNA.pdf|Hybridization of RNA blots]
[Media:Remov32P.pdf|Removal of oligolabeled probes for GeneScreen Plus membranes]
[Media:plyriprot.pdf|Yeast polyribosome preparation - Proteins]
[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions]
[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]
[Media:imblot.pdf|Immunoblotting]

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