The mRNA Decay Resource:Protocols and Resources

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 4: Line 4:
==Yeast protocols==
==Yeast protocols==
-
=From the Atkin lab= <br>
+
From the Atkin lab <br>
-
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [Media:rnapheno.pdf|Preparation of buffer saturated phenol] <br>
+
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br>
-
[Media:mrnahalf.pdf|Measurement of mRNA half lives] <br>
+
[[Media:mrnahalf.pdf|Measurement of mRNA half lives]] <br>
-
[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA] <br>
+
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
-
[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde] <br>
+
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br>
-
[Media:NorthRNA.pdf|Northern transfer of RNA] <br>
+
[[Media:NorthRNA.pdf|Northern transfer of RNA]] <br>
-
[Media:dnaprobe.pdf|Preparation of oligolabeled probes] <br>
+
[[Media:dnaprobe.pdf|Preparation of oligolabeled probes]] <br>
-
[Media:hybrdRNA.pdf|Hybridization of RNA blots] <br>
+
[[Media:hybrdRNA.pdf|Hybridization of RNA blots]] <br>
-
[Media:Remov32P.pdf|Removal of oligolabeled probes for GeneScreen <I>Plus</I> membranes] <br>
+
[[Media:Remov32P.pdf|Removal of oligolabeled probes from GeneScreen <I>Plus</I> membranes]] <br>
-
[Media:plyriprot.pdf|Yeast polyribosome preparation - Proteins] <br>
+
[[Media:plyriprot.pdf|Yeast polyribosome preparation - Proteins]] <br>
-
[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions] <br>
+
[[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions]] <br>
-
[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes] <br>
+
[[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] <br>
-
[Media:imblot.pdf|Immunoblotting] <br>
+
[[Media:imblot.pdf|Immunoblotting]] <br>

Revision as of 14:47, 17 June 2008

Home        Colleagues        Protocols and Resources        Conferences        Community Information       


Protocols

Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of mRNA half lives
Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting

Personal tools