The mRNA Decay Resource:Protocols and Resources

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(Yeast protocols)
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[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br>
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br>
[[Media:mrnahalf.pdf|Measurement of mRNA half lives]] <br>
[[Media:mrnahalf.pdf|Measurement of mRNA half lives]] <br>
 +
#Paper1 pmid=17036064
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br>
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br>
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[[Media:imblot.pdf|Immunoblotting]] <br>
[[Media:imblot.pdf|Immunoblotting]] <br>
<biblio>
<biblio>
-
#Paper1 pmid=17036064
 
</biblio><br>
</biblio><br>
'''From Fred Sherman'''
'''From Fred Sherman'''
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</biblio>
</biblio>
<br><br>
<br><br>
 +
==General RNA Protocols==
==General RNA Protocols==
<biblio>
<biblio>
#Paper1 pmid=18369981
#Paper1 pmid=18369981
</biblio>
</biblio>

Revision as of 10:43, 11 March 2009

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Protocols

Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of mRNA half lives

  1. Paper1 pmid=17036064

Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting

  1. []

From Fred Sherman

  1. Sherman F. . pmid:12073320. PubMed HubMed [Paper1]



General RNA Protocols

  1. Bajak EZ and Hagedorn CH. . pmid:18369981. PubMed HubMed [Paper1]
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