The mRNA Decay Resource:Protocols and Resources

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(Yeast protocols)
(Yeast protocols)
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'''From the Atkin lab''' <br>
'''From the Atkin lab''' <br>
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br>
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br>
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[[Media:mrnahalf.pdf|Measurement of mRNA half lives]] <br>
+
[[Media:mrnahalf.pdf|Measurement of mRNA half lives from Saccharomyces cerevisiae]] <br>
 +
<biblio>
#Paper1 pmid=17036064
#Paper1 pmid=17036064
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
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[[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] <br>
[[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] <br>
[[Media:imblot.pdf|Immunoblotting]] <br>
[[Media:imblot.pdf|Immunoblotting]] <br>
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<biblio>
+
 
</biblio><br>
</biblio><br>
'''From Fred Sherman'''
'''From Fred Sherman'''

Revision as of 10:44, 11 March 2009

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Protocols

Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of mRNA half lives from Saccharomyces cerevisiae

  1. Kebaara BW, Nielsen LE, Nickerson KW, and Atkin AL. . pmid:17036064. PubMed HubMed [Paper1]

From Fred Sherman

  1. Sherman F. . pmid:12073320. PubMed HubMed [Paper1]



General RNA Protocols

  1. Bajak EZ and Hagedorn CH. . pmid:18369981. PubMed HubMed [Paper1]
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