The mRNA Decay Resource:Protocols and Resources

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(Yeast protocols)
(Yeast protocols)
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'''From the Atkin lab''' <br>
'''From the Atkin lab''' <br>
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br>
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br>
-
[[Media:mrnahalf.pdf|Measurement of mRNA half lives from Saccharomyces cerevisiae]] <br>
+
[[Media:mrnahalf.pdf|Measurement of Saccharomyces cerevisiae mRNA half lives]] <br>
<biblio>
<biblio>
#Paper1 pmid=17036064
#Paper1 pmid=17036064
 +
</biblio>
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br>
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br>

Revision as of 11:45, 11 March 2009

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Protocols

Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of Saccharomyces cerevisiae mRNA half lives

  1. Kebaara BW, Nielsen LE, Nickerson KW, and Atkin AL. . pmid:17036064. PubMed HubMed [Paper1]

Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting

</biblio>
From Fred Sherman

  1. Sherman F. . pmid:12073320. PubMed HubMed [Paper1]



General RNA Protocols

  1. Bajak EZ and Hagedorn CH. . pmid:18369981. PubMed HubMed [Paper1]
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