The mRNA Decay Resource:Protocols and Resources: Difference between revisions
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'''From the Atkin lab''' <br> | '''From the Atkin lab''' <br> | ||
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br> | [[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br> | ||
[[Media:mrnahalf.pdf|Measurement of mRNA half lives | [[Media:mrnahalf.pdf|Measurement of Saccharomyces cerevisiae mRNA half lives]] <br> | ||
<biblio> | <biblio> | ||
#Paper1 pmid=17036064 | #Paper1 pmid=17036064 | ||
</biblio> | |||
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br> | [[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br> | ||
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br> | [[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br> | ||
Revision as of 08:45, 11 March 2009
Protocols
Yeast protocols
From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of Saccharomyces cerevisiae mRNA half lives
- Kebaara BW, Nielsen LE, Nickerson KW, and Atkin AL. Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor. Genome. 2006 Aug;49(8):894-9. DOI:10.1139/g06-046 |
Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting
</biblio>
From Fred Sherman
- Sherman F. Getting started with yeast. Methods Enzymol. 2002;350:3-41. DOI:10.1016/s0076-6879(02)50954-x |
