The mRNA Decay Resource:Protocols and Resources

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(Yeast protocols)
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[[Media:mrnahalf.pdf|Measurement of ''Saccharomyces cerevisiae'' mRNA half lives]] <br>
[[Media:mrnahalf.pdf|Measurement of ''Saccharomyces cerevisiae'' mRNA half lives]] <br>
Measurement of ''Candida albicans'' mRNA half lives:
Measurement of ''Candida albicans'' mRNA half lives:
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<biblio>
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Kebaara et al., 2006.  Determination of mRNA half-lives in ''Candida albicans'' using thiolutin as a transcription inhibitor. Genome. 49(8):894-9. [http://www.ncbi.nlm.nih.gov/pubmed?term=17036064abstract|abstract] <br>
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#Paper1 pmid=17036064
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</biblio>
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[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
[[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] <br>
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br>
[[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] <br>
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[[Media:imblot.pdf|Immunoblotting]] <br>
[[Media:imblot.pdf|Immunoblotting]] <br>
<br>
<br>
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'''From Fred Sherman'''
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'''From Fred Sherman'''<br>
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<biblio>
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Sherman, 2002. Getting started with yeast.  Methods Enzymol. 350:3-41. [http://www.ncbi.nlm.nih.gov/pubmed?term=12073320|abstract] <br>
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#Paper1 pmid=12073320
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</biblio>
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<br>
<br>
'''From Mike Culbertson'''<br>
'''From Mike Culbertson'''<br>
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Global measurement of mRNA half lives<br>
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Global measurement of mRNA half lives.  Guan et al., 2006. Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeast. PLoS Genet. 2(11):e203. Epub 2006 Oct 18. [http://www.ncbi.nlm.nih.gov/pubmed?term=17166056|abstract] <br>
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<biblio>
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<br>
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#Paper1 pmid=17166056
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</biblio>
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<br><br>
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==General RNA Protocols==
==General RNA Protocols==
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<biblio>
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Purification of capped RNAs.  Bajak and Hagedorn, 2008. Efficient 5' cap-dependent RNA purification: use in identifying and studying subsets of RNA. Methods Mol Biol. 419:147-60. [http://www.ncbi.nlm.nih.gov/pubmed?term=18369981|abstract] <br>
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#Paper1 pmid=18369981
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</biblio>
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Revision as of 14:03, 30 March 2011

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Protocols

Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of Saccharomyces cerevisiae mRNA half lives
Measurement of Candida albicans mRNA half lives: Kebaara et al., 2006. Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor. Genome. 49(8):894-9. [1]
Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting

From Fred Sherman
Sherman, 2002. Getting started with yeast. Methods Enzymol. 350:3-41. [2]

From Mike Culbertson
Global measurement of mRNA half lives. Guan et al., 2006. Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeast. PLoS Genet. 2(11):e203. Epub 2006 Oct 18. [3]

General RNA Protocols

Purification of capped RNAs. Bajak and Hagedorn, 2008. Efficient 5' cap-dependent RNA purification: use in identifying and studying subsets of RNA. Methods Mol Biol. 419:147-60. [4]

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