The mRNA Decay Resource:Protocols and Resources: Difference between revisions
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==Yeast protocols== | ==Yeast protocols== | ||
From the Atkin lab <br> | '''From the Atkin lab''' <br> | ||
[[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br> | [[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] <br> | ||
[[Media:mrnahalf.pdf|Measurement of mRNA half lives]] <br> | [[Media:mrnahalf.pdf|Measurement of mRNA half lives]] <br> | ||
Revision as of 11:56, 17 June 2008
Protocols
Yeast protocols
From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of mRNA half lives
Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting
From Fred Sherman
- Sherman F. Getting started with yeast. Methods Enzymol. 2002;350:3-41. DOI:10.1016/s0076-6879(02)50954-x |
