The mRNA Decay Resource:Protocols and Resources: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 16: | Line 16: | ||
[[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions]] <br> | [[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions]] <br> | ||
[[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] <br> | [[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] <br> | ||
[[Media:imblot.pdf|Immunoblotting]] <br><br> | [[Media:imblot.pdf|Immunoblotting]] <br> | ||
From Fred Sherman | <biblio> | ||
#Paper1 pmid=17036064 | |||
</biblio><br> | |||
'''From Fred Sherman''' | |||
<biblio> | <biblio> | ||
#Paper1 pmid=12073320 | #Paper1 pmid=12073320 | ||
Revision as of 08:15, 18 June 2008
Protocols
Yeast protocols
From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of mRNA half lives
Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting
- Kebaara BW, Nielsen LE, Nickerson KW, and Atkin AL. Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor. Genome. 2006 Aug;49(8):894-9. DOI:10.1139/g06-046 |
From Fred Sherman
- Sherman F. Getting started with yeast. Methods Enzymol. 2002;350:3-41. DOI:10.1016/s0076-6879(02)50954-x |
