The mRNA Decay Resource:Protocols and Resources

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Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of mRNA half lives

1. Paper1 pmid=17036064

Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting

From Fred Sherman

2. Sherman F. Getting started with yeast. Methods Enzymol. 2002;350:3-41. DOI:10.1016/s0076-6879(02)50954-x |

General RNA Protocols

2. Bajak EZ and Hagedorn CH. Efficient 5' cap-dependent RNA purification : use in identifying and studying subsets of RNA. Methods Mol Biol. 2008;419:147-60. DOI:10.1007/978-1-59745-033-1_10 |