The mRNA Decay Resource:Protocols and Resources

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Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of Saccharomyces cerevisiae mRNA half lives
Measurement of Candida albicans mRNA half lives:

1. Kebaara BW, Nielsen LE, Nickerson KW, and Atkin AL. Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor. Genome. 2006 Aug;49(8):894-9. DOI:10.1139/g06-046 |

Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
Immunoblotting

From Fred Sherman

1. Sherman F. Getting started with yeast. Methods Enzymol. 2002;350:3-41. DOI:10.1016/s0076-6879(02)50954-x |

From Mike Culbertson
Global measurement of mRNA half lives

1. Guan Q, Zheng W, Tang S, Liu X, Zinkel RA, Tsui KW, Yandell BS, and Culbertson MR. Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeast. PLoS Genet. 2006 Nov 24;2(11):e203. DOI:10.1371/journal.pgen.0020203 |

General RNA Protocols

1. Bajak EZ and Hagedorn CH. Efficient 5' cap-dependent RNA purification : use in identifying and studying subsets of RNA. Methods Mol Biol. 2008;419:147-60. DOI:10.1007/978-1-59745-033-1_10 |