The mRNA Decay Resource:Protocols and Resources

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Protocols

Yeast protocols from the Atkin lab

<A HREF="RNAextrc.pdf" TARGET="_blank">Yeast RNA extraction</A>

   
Support Protocol: <A HREF="rnapheno.pdf" TARGET="_blank">Preparation of buffer saturated phenol</A>
   
   
<A HREF="mrnahalf.pdf">Measurement of mRNA half lives</A>
<A HREF="PlyriRNA.pdf">Yeast polyribosome preparation - RNA</A>
<A HREF="RNAgels.pdf">Electrophoresis of RNA through gels containing formaldehyde</A>
<A HREF="NorthRNA.pdf">Northern transfer of RNA</A>
<A HREF="dnaprobe.pdf">Preparation of oligolabeled probes</A>
   
<A HREF="hybrdRNA.pdf">Hybridization of RNA blots</A>
<A HREF="Remov32P.pdf">Removal of oligolabeled probes for GeneScreen Plus membranes</A>
<A HREF="plyriprot.pdf">Yeast polyribosome preparation - Proteins</A>
<A HREF="protgel.pdf">Gel electrophoresis of proteins in denaturing conditions</A>
<A HREF="protran.pdf">Electrotransfer of proteins from polyacrylamide gels to membranes</A>
   
<A HREF="imblot.pdf">Immunoblotting</A>
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