The mRNA Decay Resource:Protocols and Resources

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Protocols

Yeast protocols

From the Atkin lab
Total RNA extraction Support Protocol: [Media:rnapheno.pd| Preparation of buffer saturated phenol]
[Media:mrnahalf.pdf Measurement of mRNA half lives]
[Media:PlyriRNA.pdf Yeast polyribosome preparation for RNA]
[Media:RNAgels.pdf Electrophoresis of RNA through gels containing formaldehyde]
[Media:NorthRNA.pdf Northern transfer of RNA]
[Media:dnaprobe.pdf Preparation of oligolabeled probes]
[Media:hybrdRNA.pdf Hybridization of RNA blots]
[Media:Remov32P.pdf Removal of oligolabeled probes for GeneScreen Plus membranes]
[Media:plyriprot.pdf Yeast polyribosome preparation - Proteins]
[Media:protgel.pdf Gel electrophoresis of proteins in denaturing conditions]
[Media:protran.pdf Electrotransfer of proteins from polyacrylamide gels to membranes]
[Media:imblot.pdf Immunoblotting]

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