The mRNA Decay Resource:Protocols and Resources
From the Atkin lab
Total RNA extraction Support Protocol: Preparation of buffer saturated phenol
Measurement of Saccharomyces cerevisiae mRNA half lives
Measurement of Candida albicans mRNA half lives:
- Kebaara BW, Nielsen LE, Nickerson KW, and Atkin AL. . pmid:17036064.
Yeast polyribosome preparation for RNA
Electrophoresis of RNA through gels containing formaldehyde
Northern transfer of RNA
Preparation of oligolabeled probes
Hybridization of RNA blots
Removal of oligolabeled probes from GeneScreen Plus membranes
Yeast polyribosome preparation - Proteins
Gel electrophoresis of proteins in denaturing conditions
Electrotransfer of proteins from polyacrylamide gels to membranes
From Fred Sherman
- Sherman F. . pmid:12073320.
From Mike Culbertson Global measurement of mRNA half lives
General RNA Protocols
- Bajak EZ and Hagedorn CH. . pmid:18369981.