Tiffany Guo: Difference between revisions
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| Line 24: | Line 24: | ||
| III | | III | ||
| phage tail protein (5 copies) | | phage tail protein (5 copies) | ||
| | | modify end of protein so that it can bind to other cells (and infect other cells) besides E. Coli | ||
|- | |- | ||
| IV | | IV | ||
| Line 30: | Line 30: | ||
| | | | ||
|- | |- | ||
| V | | V | ||
| binds ssDNA | | binds ssDNA | ||
| | | allow it to sequester double stranded DNA also, then it can be used as a vector for infecting cells with desired DNA fragments. | ||
|- | |- | ||
| VI | | VI | ||
| phage tail protein (5 copies) | | phage tail protein (5 copies) | ||
| | | delete to learn more about its function. | ||
|- | |- | ||
| VII | | VII | ||
| phage head protein (5 copies) | | phage head protein (5 copies) | ||
| | | delete to learn more about its function. | ||
|- | |- | ||
| VIII | | VIII | ||
| phage coat protein (2700 copies) | | phage coat protein (2700 copies) | ||
| | | tag it (perhaps with flourescence) to learn more about phage coat assembly | ||
|- | |- | ||
| IX | | IX | ||
| phage head protein (5 copies) | | phage head protein (5 copies) | ||
| | | | ||
|- | |- | ||
| X | | X | ||
| DNA replication | | DNA replication | ||
| | | make pX more active so that more + strands will accumulate, allowing the host cell to produce even more phages. | ||
|- | |- | ||
| XI | | XI | ||
Revision as of 20:53, 13 February 2007
About me
Name: Tiffany Guo
Course/Minor: 20/5
Year of Graduation: 2009
Email: tiffguo
Current Classes: 5.03, 6.00, 20.109, 21F.108
Module 1: Genome Engineering
Day 1: Start up Genome Engineering
| protein | function | re-engineering ideas |
|---|---|---|
| I | assembly | |
| II | replication of DNA + strand | |
| III | phage tail protein (5 copies) | modify end of protein so that it can bind to other cells (and infect other cells) besides E. Coli |
| IV | assembly | |
| V | binds ssDNA | allow it to sequester double stranded DNA also, then it can be used as a vector for infecting cells with desired DNA fragments. |
| VI | phage tail protein (5 copies) | delete to learn more about its function. |
| VII | phage head protein (5 copies) | delete to learn more about its function. |
| VIII | phage coat protein (2700 copies) | tag it (perhaps with flourescence) to learn more about phage coat assembly |
| IX | phage head protein (5 copies) | |
| X | DNA replication | make pX more active so that more + strands will accumulate, allowing the host cell to produce even more phages. |
| XI | assembly |
