Tiffany Guo: Difference between revisions
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| I | | I | ||
| assembly | | assembly | ||
| | | modify gene to that multiple channels can be made, and thus mature phage secretion is accelerated | ||
|- | |- | ||
| II | | II | ||
| replication of DNA + strand | | replication of DNA + strand | ||
| | | modify so it can nick foreign DNA (e.g. the E. Coli's) and the phage can replicate and package a portion of the host DNA | ||
|- | |- | ||
| III | | III | ||
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| IV | | IV | ||
| assembly | | assembly | ||
| | | modify gene to that multiple channels can be made, and thus mature phage secretion is accelerated | ||
|- | |- | ||
| V | | V | ||
| Line 48: | Line 48: | ||
| IX | | IX | ||
| phage head protein (5 copies) | | phage head protein (5 copies) | ||
| | | modify so that it can act similarly to pIII | ||
|- | |- | ||
| X | | X | ||
| Line 56: | Line 56: | ||
| XI | | XI | ||
| assembly | | assembly | ||
| | | modify gene to that multiple channels can be made, and thus mature phage secretion is accelerated | ||
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Revision as of 21:06, 13 February 2007
About me
Name: Tiffany Guo
Course/Minor: 20/5
Year of Graduation: 2009
Email: tiffguo
Current Classes: 5.03, 6.00, 20.109, 21F.108
Module 1: Genome Engineering
Day 1: Start up Genome Engineering
| protein | function | re-engineering ideas |
|---|---|---|
| I | assembly | modify gene to that multiple channels can be made, and thus mature phage secretion is accelerated |
| II | replication of DNA + strand | modify so it can nick foreign DNA (e.g. the E. Coli's) and the phage can replicate and package a portion of the host DNA |
| III | phage tail protein (5 copies) | modify end of protein so that it can bind to other cells (and infect other cells) besides E. Coli |
| IV | assembly | modify gene to that multiple channels can be made, and thus mature phage secretion is accelerated |
| V | binds ssDNA | allow it to sequester double stranded DNA also, then it can be used as a vector for infecting cells with desired DNA fragments. |
| VI | phage tail protein (5 copies) | delete to learn more about its function. |
| VII | phage head protein (5 copies) | delete to learn more about its function. |
| VIII | phage coat protein (2700 copies) | tag it (perhaps with flourescence) to learn more about phage coat assembly |
| IX | phage head protein (5 copies) | modify so that it can act similarly to pIII |
| X | DNA replication | make pX more active so that more + strands will accumulate, allowing the host cell to produce even more phages. |
| XI | assembly | modify gene to that multiple channels can be made, and thus mature phage secretion is accelerated |
