Tk:lab stuff: Difference between revisions

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Line 9: Line 9:
* (some 8 well pipettors)
* (some 8 well pipettors)
* Dry block heater
* Dry block heater
* gloves
* Foam tube holders
* Foam tube holders
* water filled trays for melting reagents
* water filled trays for melting reagents
Line 104: Line 105:
* sponges
* sponges
* mop
* mop
* hazardous material gloves
* large platic bins


==Incubators==
==Incubators==
Line 153: Line 157:
* Pipets 10 ml glass
* Pipets 10 ml glass
* Pasteur pipets
* Pasteur pipets
* gloves
* Microtiter plates
* Microtiter plates
* Lids for microtiter plates
* Lids for microtiter plates
Line 176: Line 181:
* paper
* paper
* Reagent reservoirs
* Reagent reservoirs
* gas cartridges for fireboys
* clean air squirters


==Computers==
==Computers==
Line 243: Line 251:
==Microwave==
==Microwave==
* domestic microwave large enough for 1 liter bottles
* domestic microwave large enough for 1 liter bottles
==Electrophoresis==
* gel boxes
* casting trays
* gel trays
* combs 8, 12, 15
* power supplies
* Sybr Safe dye
* DNA ladder (NEB 2 log ladder)
* loading dye
** ficol
** orange G
* paper towels
* Clare dark reader
* Razor blades
* while light box
* camera stand
* saran wrap dispenser
* TAE jugs
* E Gel setup
* supply of E-Gels
* Gel imager
** white trans/refl, UV 305, 365 trans
** also western luminescent imager
* hybridization oven
* UV exposure system
* bag sealer
* 6 x 10 inch platic bags (sealable)
==Glassware==
* media bottles
* Round 100 ml
* Square 250, 500, 1000 ml
* round 2 liter
* extra caps
** red hot caps
** holed septum caps
** extra seal rings (white, red)
* Grey vented caps
* Erlenmeyer flasks, 250, 500, 1000, 2000 ml with 45 ml lids
* amber 100 ml round bottles
* Graduates (plastic) 10, 25, 50, 100, 250, 1000, 4000 ml
* separatory funnels
* funnels
* plastic bottles
* Scoops
* buchner funnels
* corks
* cork borer set
* glass tubing (soft, pyrex)
* capillary tubing
* chemical bottles

Revision as of 22:23, 14 April 2007

At the bench

  • mini centrifuge
  • fireboy
  • vortexer
  • Pipettor set
  • shelf rack for pipttors
  • Dumont Pipet
  • (some 8 well pipettors)
  • Dry block heater
  • gloves
  • Foam tube holders
  • water filled trays for melting reagents
  • ice buckets
  • pipet holder
  • bench light
  • paper towels
  • computer
  • calculator
  • lab notebook
  • 80 hole eppendorf tube holders
  • 96 hole PCCR tube holders
  • 16 ml centrifuge tubes
  • 60 ml centrifuge tubes
  • test tube rack (epoxy, also for 15 ml centrifuge tubes)
  • rack for 50 ml centrifuge tubes
  • sharps disposal bin
  • containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
  • containser for cryotubes, 0.5ml screw cap tubes


  • drawer
    • loop
    • tweezers
    • razor blades
    • PCR 8 well strips
    • caps for PCR strips
    • pens
    • lab markers
    • lab tape
    • label maker
    • scotch tape
    • petri-stickers

==autoclave--

  • Yamato autoclave (or two)
  • autoclave tape
  • 1 liter beakers for water
  • drain pans
  • autoclave verrification tests
  • hot gloves


dishwasher

  • lab dish washer
  • alconox dishwasher soap
  • basket for caps
  • carts for bottles


fume hood

  • vacuum system
  • bottle top 0.2 u filters
  • Pierce blow dry system


Plate pouring

  • petri dishes
  • lab tape
  • water bath
  • antibiotics


Scale

  • weigh boats
  • dispsible spatulas
  • scale
  • brush
  • calibration weights


pH meter

  • Beckman pH meter
  • Tris friendly electrode, 5mm
  • calibration solutions
  • pH storage solution
  • pH filling solution
  • magnetic stir bars
  • magnetic stirrer
  • pH paper


Sink

  • paper towels
  • Alcoscrub
  • 70% Ethanol
  • Bleach
  • Liquid hand soap
  • Mat for bottom of sink
  • water bath
  • Evaporation beads for water bath
  • Copper sulfate mold prevention for water bath
  • Acid Base Biohazard spill kits
  • buckets
  • sponges
  • mop
  • hazardous material gloves
  • large platic bins


Incubators

  • New Brunswick shaker
    • various size holders
    • screws, screwdrivers
    • rack for tubes
  • VWR 37 and 30 incubators
  • rotators
  • shakers
  • small shaking incubator (Eppendorf)


PCR machines

  • Opticon or equivalent
  • dual block 0.5 ml alpha unit
  • 8 well strips and caps
  • some plates


Spectrophotometer

  • Nanodrop
  • kimwipes
  • PC for software


Plate Reader

Vacuum evaporator

Freeze Dryer

  • torch
  • serum bottles
  • caps
  • lyophilizer freeze shelf / capping station


Water purification sysem

Supplies

  • Petri dishes, 100mm, 60 mm
  • Pipet tips
  • Pipets 10, 25, 50 ml
  • Pipets 10 ml glass
  • Pasteur pipets
  • gloves
  • Microtiter plates
  • Lids for microtiter plates
  • Tape for label makers
  • hypodermics & needles 1, 3, 10, 20, 60 ml
  • 2.0, 1.7, 0.5 thick/thin, screw cap 2.0, 0.5, cryotubes
  • 2059 14 ml test tubes
  • 15 ml centrifuge tubes
  • 50 ml centrifuge tubes
  • foam insert freezer boxes
  • stainless steel freezer shelves
  • paper towels
  • sharps containers
  • autoclave bags
  • biohazard bins
  • hazardous waste labels
  • lab tape
  • autoclave tape
  • scotch tape
  • lab markers
  • pens
  • lab notebooks
  • paper
  • Reagent reservoirs
  • gas cartridges for fireboys
  • clean air squirters


Computers

  • fax machine
  • printer (color)
  • internet access
  • scanner
  • video recorder
  • camera
  • software


Electroporator

  • zapper
  • cuvettes


Bead beater

  • beater
  • Beads


Sonicator

Protein Gels

  • power supply
  • gel boxes
  • blotter
  • tilt table shaker
  • trays
  • Coumassie stain
  • Gel opener


RNA bench

  • distinguished pipettors
  • RNAse away
  • distinguished gel boxes
  • DEPC


Ice maker

  • Ice buckets
  • scoops


Dry ice container

  • with wheels


Freezers

  • -80 upright with freezer drawers for boxes
  • -20
  • chromatography style +4
  • small under counter freezers and fridges


Centrifuges

  • Large Eppendorf 5810R
    • fixed angle rotor with inserts for 15 and 50 ml tubes
    • swing rotor with plate, centrifuge tube for 15, 50, 300 ml tubes
    • all inserts required
  • Small benchtop 5817R with 30 position rotor


Microwave

  • domestic microwave large enough for 1 liter bottles


Electrophoresis

  • gel boxes
  • casting trays
  • gel trays
  • combs 8, 12, 15
  • power supplies
  • Sybr Safe dye
  • DNA ladder (NEB 2 log ladder)
  • loading dye
    • ficol
    • orange G
  • paper towels
  • Clare dark reader
  • Razor blades
  • while light box
  • camera stand
  • saran wrap dispenser
  • TAE jugs
  • E Gel setup
  • supply of E-Gels
  • Gel imager
    • white trans/refl, UV 305, 365 trans
    • also western luminescent imager
  • hybridization oven
  • UV exposure system
  • bag sealer
  • 6 x 10 inch platic bags (sealable)


Glassware

  • media bottles
  • Round 100 ml
  • Square 250, 500, 1000 ml
  • round 2 liter
  • extra caps
    • red hot caps
    • holed septum caps
    • extra seal rings (white, red)
  • Grey vented caps
  • Erlenmeyer flasks, 250, 500, 1000, 2000 ml with 45 ml lids
  • amber 100 ml round bottles
  • Graduates (plastic) 10, 25, 50, 100, 250, 1000, 4000 ml
  • separatory funnels
  • funnels
  • plastic bottles
  • Scoops
  • buchner funnels
  • corks
  • cork borer set
  • glass tubing (soft, pyrex)
  • capillary tubing
  • chemical bottles