Tk:lab stuff

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(At the bench)
Current revision (14:57, 6 December 2007) (view source)
(Large equipment)
 
(29 intermediate revisions not shown.)
Line 6: Line 6:
* Pipettor set
* Pipettor set
* shelf rack for pipttors
* shelf rack for pipttors
-
* Dumont Pipet
+
* Drummond Pipettor
 +
* power strips
 +
* timer
* (some 8 well pipettors)
* (some 8 well pipettors)
* Dry block heater
* Dry block heater
Line 25: Line 27:
* test tube rack (epoxy, also for 15 ml centrifuge tubes)
* test tube rack (epoxy, also for 15 ml centrifuge tubes)
* rack for 50 ml centrifuge tubes
* rack for 50 ml centrifuge tubes
 +
* floaters
* sharps disposal bin
* sharps disposal bin
* containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
* containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
-
* containser for cryotubes, 0.5ml screw cap tubes
+
* container for cryotubes, 0.5ml screw cap tubes
-
 
+
* lab stool
 +
* lab coat
 +
* glass beads
 +
* toothpicks
 +
* spreader
 +
* Alcohol for spreader
 +
* parfilm
 +
* wash bottles (EtOH, water)
* drawer
* drawer
Line 42: Line 52:
** scotch tape
** scotch tape
** petri-stickers
** petri-stickers
-
 
+
** stapler
 +
** scissors
==autoclave==
==autoclave==
Line 49: Line 60:
* 1 liter beakers for water
* 1 liter beakers for water
* drain pans
* drain pans
-
* autoclave verrification tests
+
* autoclave verification tests
* hot gloves
* hot gloves
 +
* pouches for sterilization
 +
* Aluminum foil sheets
==dishwasher==
==dishwasher==
Line 74: Line 87:
==Scale==
==Scale==
* weigh boats
* weigh boats
 +
* weigh paper
* dispsible spatulas
* dispsible spatulas
* scale
* scale
* brush
* brush
* calibration weights
* calibration weights
-
 
==pH meter==
==pH meter==
Line 88: Line 101:
* magnetic stir bars
* magnetic stir bars
* magnetic stirrer
* magnetic stirrer
 +
* stir bar retrieval tool
* pH paper
* pH paper
-
 
==Sink==
==Sink==
Line 95: Line 108:
* Alcoscrub
* Alcoscrub
* 70% Ethanol
* 70% Ethanol
 +
* Curel
* Bleach
* Bleach
* Liquid hand soap
* Liquid hand soap
Line 107: Line 121:
* hazardous material gloves
* hazardous material gloves
* large platic bins
* large platic bins
-
 
==Incubators==
==Incubators==
Line 144: Line 157:
* caps
* caps
* lyophilizer freeze shelf / capping station
* lyophilizer freeze shelf / capping station
-
 
+
* aluminum seal capper
==Water purification sysem==
==Water purification sysem==
Line 183: Line 196:
* gas cartridges for fireboys
* gas cartridges for fireboys
* clean air squirters
* clean air squirters
-
 
+
* staples (large, small)
 +
* stapler removal tools
 +
* hole punch
==Computers==
==Computers==
Line 193: Line 208:
* camera
* camera
* software
* software
-
 
+
* copier
 +
* binder
==Electroporator==
==Electroporator==
Line 216: Line 232:
* Coumassie stain
* Coumassie stain
* Gel opener
* Gel opener
-
 
+
* drying frames
==RNA bench==
==RNA bench==
Line 280: Line 296:
* bag sealer
* bag sealer
* 6 x 10 inch platic bags (sealable)
* 6 x 10 inch platic bags (sealable)
-
 
==Glassware==
==Glassware==
Line 306: Line 321:
* chemical bottles
* chemical bottles
* volumetric flasks
* volumetric flasks
 +
* sidearm flasks
 +
* glassware wash brushes
 +
* glassware cleaning beads
 +
* desiccator (w/ vacuum sidearm)
 +
* beakers
 +
** handles
 +
** glass
 +
** plastic, no handle
 +
* beaker tongs
 +
* hybridization bottles
 +
 +
 +
==Miscellaneous==
 +
* tubing
 +
* hazardous waste secondary containers
 +
* hand vacuum pumps
 +
* thermometers
 +
* replica plating tool
 +
* biohazard labels
 +
* chemical safety hazard labels
 +
 +
==TLC system==
 +
* container
 +
* top
 +
* TLC membranes
 +
 +
==Filters==
 +
* Whatman paper
 +
** sheets
 +
** circles
 +
* Microcon columns
 +
* Blotting paper
 +
* PVDF membranes
 +
* Nitrocellulose membranes
 +
* perforated sheets (hybridization)
 +
* Bottle top filters (0.2 u)
 +
* empty columns
 +
** spin columns
 +
** flow columns
 +
** luer valve
 +
 +
==Large equipment==
 +
* sequencer
 +
* synthesizer
 +
* robot
 +
* mass spec
 +
* plate reader
 +
** luminescent
 +
** OD
 +
** fluorescent
 +
* quantitative PCR
 +
* PCR cycler
 +
* nanodrop (both spec and fluorescent)
 +
* CD
 +
* microcalorimetry
 +
* HPLC / FPLC
 +
* capillary electrophoresis
 +
* -80 freezer
 +
* -20 freezer
 +
* +4 chromatography fridge
 +
* Flaked ice maker
 +
* dishwasher
 +
* autoclaves (2)
 +
* DI water system
 +
* Eppendorf 5810R
 +
* Eppendorf 5817R
 +
* pH meter
 +
* freeze dryer
 +
* vacuum evaporator
 +
* New Brunswick shaker/incubator
 +
* backup power
 +
* microscope

Current revision

Contents

At the bench

  • mini centrifuge
  • fireboy
  • vortexer
  • Pipettor set
  • shelf rack for pipttors
  • Drummond Pipettor
  • power strips
  • timer
  • (some 8 well pipettors)
  • Dry block heater
  • gloves
  • Foam tube holders
  • water filled trays for melting reagents
  • ice buckets
  • pipet holder
  • bench light
  • paper towels
  • computer
  • calculator
  • lab notebook
  • 80 hole eppendorf tube holders
  • 96 hole PCCR tube holders
  • 16 ml centrifuge tubes
  • 60 ml centrifuge tubes
  • test tube rack (epoxy, also for 15 ml centrifuge tubes)
  • rack for 50 ml centrifuge tubes
  • floaters
  • sharps disposal bin
  • containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
  • container for cryotubes, 0.5ml screw cap tubes
  • lab stool
  • lab coat
  • glass beads
  • toothpicks
  • spreader
  • Alcohol for spreader
  • parfilm
  • wash bottles (EtOH, water)
  • drawer
    • loop
    • tweezers
    • razor blades
    • PCR 8 well strips
    • caps for PCR strips
    • pens
    • lab markers
    • lab tape
    • label maker
    • scotch tape
    • petri-stickers
    • stapler
    • scissors

autoclave

  • Yamato autoclave (or two)
  • autoclave tape
  • 1 liter beakers for water
  • drain pans
  • autoclave verification tests
  • hot gloves
  • pouches for sterilization
  • Aluminum foil sheets

dishwasher

  • lab dish washer
  • alconox dishwasher soap
  • basket for caps
  • carts for bottles


fume hood

  • vacuum system
  • bottle top 0.2 u filters
  • Pierce blow dry system


Plate pouring

  • petri dishes
  • lab tape
  • water bath
  • antibiotics


Scale

  • weigh boats
  • weigh paper
  • dispsible spatulas
  • scale
  • brush
  • calibration weights

pH meter

  • Beckman pH meter
  • Tris friendly electrode, 5mm
  • calibration solutions
  • pH storage solution
  • pH filling solution
  • magnetic stir bars
  • magnetic stirrer
  • stir bar retrieval tool
  • pH paper

Sink

  • paper towels
  • Alcoscrub
  • 70% Ethanol
  • Curel
  • Bleach
  • Liquid hand soap
  • Mat for bottom of sink
  • water bath
  • Evaporation beads for water bath
  • Copper sulfate mold prevention for water bath
  • Acid Base Biohazard spill kits
  • buckets
  • sponges
  • mop
  • hazardous material gloves
  • large platic bins

Incubators

  • New Brunswick shaker
    • various size holders
    • screws, screwdrivers
    • rack for tubes
  • VWR 37 and 30 incubators
  • rotators
  • shakers
  • small shaking incubator (Eppendorf)


PCR machines

  • Opticon or equivalent
  • dual block 0.5 ml alpha unit
  • 8 well strips and caps
  • some plates


Spectrophotometer

  • Nanodrop
  • kimwipes
  • PC for software


Plate Reader

Vacuum evaporator

Freeze Dryer

  • torch
  • serum bottles
  • caps
  • lyophilizer freeze shelf / capping station
  • aluminum seal capper

Water purification sysem

Supplies

  • Petri dishes, 100mm, 60 mm
  • Pipet tips
  • Pipets 10, 25, 50 ml
  • Pipets 10 ml glass
  • Pasteur pipets
  • gloves
  • Microtiter plates
  • Lids for microtiter plates
  • Tape for label makers
  • hypodermics & needles 1, 3, 10, 20, 60 ml
  • 2.0, 1.7, 0.5 thick/thin, screw cap 2.0, 0.5, cryotubes
  • 2059 14 ml test tubes
  • 15 ml centrifuge tubes
  • 50 ml centrifuge tubes
  • foam insert freezer boxes
  • stainless steel freezer shelves
  • paper towels
  • sharps containers
  • autoclave bags
  • biohazard bins
  • hazardous waste labels
  • lab tape
  • autoclave tape
  • scotch tape
  • lab markers
  • pens
  • lab notebooks
  • paper
  • Reagent reservoirs
  • gas cartridges for fireboys
  • clean air squirters
  • staples (large, small)
  • stapler removal tools
  • hole punch

Computers

  • fax machine
  • printer (color)
  • internet access
  • scanner
  • video recorder
  • camera
  • software
  • copier
  • binder

Electroporator

  • zapper
  • cuvettes


Bead beater

  • beater
  • Beads


Sonicator

Protein Gels

  • power supply
  • gel boxes
  • blotter
  • tilt table shaker
  • trays
  • Coumassie stain
  • Gel opener
  • drying frames

RNA bench

  • distinguished pipettors
  • RNAse away
  • distinguished gel boxes
  • DEPC


Ice maker

  • Ice buckets
  • scoops


Dry ice container

  • with wheels


Freezers

  • -80 upright with freezer drawers for boxes
  • -20
  • chromatography style +4
  • small under counter freezers and fridges


Centrifuges

  • Large Eppendorf 5810R
    • fixed angle rotor with inserts for 15 and 50 ml tubes
    • swing rotor with plate, centrifuge tube for 15, 50, 300 ml tubes
    • all inserts required
  • Small benchtop 5817R with 30 position rotor


Microwave

  • domestic microwave large enough for 1 liter bottles


Electrophoresis

  • gel boxes
  • casting trays
  • gel trays
  • combs 8, 12, 15
  • power supplies
  • Sybr Safe dye
  • DNA ladder (NEB 2 log ladder)
  • loading dye
    • ficol
    • orange G
  • paper towels
  • Clare dark reader
  • Razor blades
  • while light box
  • camera stand
  • saran wrap dispenser
  • TAE jugs
  • E Gel setup
  • supply of E-Gels
  • Gel imager
    • white trans/refl, UV 305, 365 trans
    • also western luminescent imager
  • hybridization oven
  • UV exposure system
  • bag sealer
  • 6 x 10 inch platic bags (sealable)

Glassware

  • media bottles
  • Round 100 ml
  • Square 250, 500, 1000 ml
  • round 2 liter
  • extra caps
    • red hot caps
    • holed septum caps
    • extra seal rings (white, red)
  • Grey vented caps
  • Erlenmeyer flasks, 250, 500, 1000, 2000 ml with 45 ml lids
  • amber 100 ml round bottles
  • Graduates (plastic) 10, 25, 50, 100, 250, 1000, 4000 ml
  • separatory funnels
  • funnels
  • plastic bottles
  • Scoops
  • buchner funnels
  • corks
  • cork borer set
  • glass tubing (soft, pyrex)
  • capillary tubing
  • chemical bottles
  • volumetric flasks
  • sidearm flasks
  • glassware wash brushes
  • glassware cleaning beads
  • desiccator (w/ vacuum sidearm)
  • beakers
    • handles
    • glass
    • plastic, no handle
  • beaker tongs
  • hybridization bottles


Miscellaneous

  • tubing
  • hazardous waste secondary containers
  • hand vacuum pumps
  • thermometers
  • replica plating tool
  • biohazard labels
  • chemical safety hazard labels

TLC system

  • container
  • top
  • TLC membranes

Filters

  • Whatman paper
    • sheets
    • circles
  • Microcon columns
  • Blotting paper
  • PVDF membranes
  • Nitrocellulose membranes
  • perforated sheets (hybridization)
  • Bottle top filters (0.2 u)
  • empty columns
    • spin columns
    • flow columns
    • luer valve

Large equipment

  • sequencer
  • synthesizer
  • robot
  • mass spec
  • plate reader
    • luminescent
    • OD
    • fluorescent
  • quantitative PCR
  • PCR cycler
  • nanodrop (both spec and fluorescent)
  • CD
  • microcalorimetry
  • HPLC / FPLC
  • capillary electrophoresis
  • -80 freezer
  • -20 freezer
  • +4 chromatography fridge
  • Flaked ice maker
  • dishwasher
  • autoclaves (2)
  • DI water system
  • Eppendorf 5810R
  • Eppendorf 5817R
  • pH meter
  • freeze dryer
  • vacuum evaporator
  • New Brunswick shaker/incubator
  • backup power
  • microscope
Personal tools