Tk:video protocols: Difference between revisions
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** OD measurements | ** OD measurements | ||
** phases of growth | ** phases of growth | ||
* Receiving and sending strains and plasmids | * Receiving and sending strains and plasmids | ||
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** sending stabs | ** sending stabs | ||
** sending dry ice samples | ** sending dry ice samples | ||
* Labels and lab notebooks | * Labels and lab notebooks | ||
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** visualizing the bands | ** visualizing the bands | ||
** UV exposure issues | ** UV exposure issues | ||
* Cutting DNA from agarose gels | * Cutting DNA from agarose gels |
Latest revision as of 18:22, 10 November 2006
- Making plates / Media
- antibiotics
- weighing
- autoclaving
- pH
- water bath / prevention of stuff growing
- sterile technique
- material
- sterile filtration
- defined media
- Growing cultures
- spreaders / glass beads
- biosafety
- rotators
- aeration
- pH
- indicators
- temperature
- OD measurements
- phases of growth
- Receiving and sending strains and plasmids
- recovering plasmids from spotted or dried DNA
- growing stab cultures
- preserving cultures in glycerol
- preserving cultures with stabs
- preserving cultures on plates
- sending DNA
- sending stabs
- sending dry ice samples
- Labels and lab notebooks
- dated / initialed labels
- lab notebooks
- Agarose gels
- percentage choice
- buffer choice
- microwaving the gel
- adding dye
- pouring the gels
- bottle cleanup
- running the gel
- buffer choice
- adding dye to the buffer
- running time
- other kinds of agarose
- loading dye
- dna markers
- visualizing the bands
- UV exposure issues
- Cutting DNA from agarose gels
- UV exposure issues
- weighing gel samples
- isolation of DNA from gel slices
- PCR reactions
- primer design
- reaction components
- setting up the reaction
- choice of cycling conditions
- hot lids