Tk:wanted parts: Difference between revisions
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** Thrombin cleavage domain | ** Thrombin cleavage domain | ||
*** LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved | *** LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved | ||
** Calmodulin tag | |||
** S-tag | |||
** GFP protein tags | |||
* protein solubility | * protein solubility |
Revision as of 07:55, 18 July 2008
- Expression biobrick plasmids
- T7 promoter
- T3
- SP6
- T7 RNA polymerase containing strains
- also T3, SP6 What are the patent issues on these?
- xre protein, promoters (subtilis)
- antibiotic cassettes
- Apramycin
- Trimethoprim
- Transposase genes
- R6 origin plasmids
- chomosomal integration fragments
- choice of locations on the chromosome
- cell killing enzymes
- VSVG
- protein export tags
- protein cleavage domains
- protein purification tags, antibody epitopes
- FLAG
- DYKDDDDK, pmid 8770418
- FLAG Epitope description
- c-MYC
- EQKLISEEDL
- HA
- YPYDVPDYA
- 6HIS
- HHHHHH
- Strep
- AWRHPQFGG
- Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
- GST (n-terminal)
- Chitin binding
- BCCP biotin carboxyl carrier protein
- VSVG
- Thrombin cleavage domain
- LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
- Calmodulin tag
- S-tag
- GFP protein tags
- FLAG
- protein solubility
- maltose binding protein
- Thioredoxin
- protein splicing domains (inteins)
- surface display proteins
- LPP/OmpA
- Neisseria IgA1
- New fluorescent proteins
- EBFP2 blue
- new red monomers
- luminescent reporters
- renilla
- photuris
- bacterial (Photorhabdus)
- dual reporter renilla/photuris being done with John Cumbers (Brown)
- dual reporter red/green beetle luciferase dual color
- enzymatic reporters
- HRP protein
- lacZ
- Alkaline phosphatase
- Zinc finger domains
- Repair enzymes
- UDG
- mutS, mutC
- RNA sensor domains for all small molecules
- negative selection markers
- sacB
- pheS mutated
- toxic proteins
- ccdB and similar
- Mesoplasma florum genes
- recT, recE
- GFP