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Mode of Action

Trimethoprim inhibits folate metabolism. Addition of folate to the medium can counteract trimethoprim.

Resistance Gene

Dihydrofolate Reductase (DHFR) is the gene counteracting trimethoprim.

Transposon Mutagenesis

Epicentre provides a Tn5 transposon system containing the DHFR gene for random knockouts of genes in bacteria.

Working concentrations and stock solutions

Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble, and very difficult to use as a stock solution. Addition of the powder is the easiest approach.

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