Tris

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 2: Line 2:
* The pKa of Tris is 8.3
* The pKa of Tris is 8.3
 +
* dpK<sub>a</sub>/dT is -.028
 +
* A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
 +
* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.

Revision as of 16:36, 12 September 2006

Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane.

  • The pKa of Tris is 8.3
  • dpKa/dT is -.028
  • A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
  • Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
Personal tools