Tris
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* The pKa of Tris is 8.3 | * The pKa of Tris is 8.3 | ||
| + | * dpK<sub>a</sub>/dT is -.028 | ||
| + | * A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C | ||
| + | * Dilution by a factor of 10 will reduce the pH by 0.1 pH units. | ||
Revision as of 17:36, 12 September 2006
Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane.
- The pKa of Tris is 8.3
- dpKa/dT is -.028
- A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
- Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
