Tris: Difference between revisions
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Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane. | Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>). | ||
* The formula weight is 121.14 | |||
* The pKa of Tris is 8.3 | * The pKa of Tris is 8.3 | ||
* dpK<sub>a</sub>/dT is -.028 | * dpK<sub>a</sub>/dT is -.028 | ||
Revision as of 17:30, 11 April 2007
Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3).
- The formula weight is 121.14
- The pKa of Tris is 8.3
- dpKa/dT is -.028
- A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
- Note that adding HCl during titration will affect the temperature of the solution
- Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
