Triton X-100

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from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
* store at room temperature protected from bright light
* store at room temperature protected from bright light
-
* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
+
* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated  
* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated  
hydroxytoluene per 500 moles of detergent to prevent autoxidation  
hydroxytoluene per 500 moles of detergent to prevent autoxidation  
-
+
 
 +
== Tips ==
 +
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
 +
* The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
 +
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
 +
* When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
 +
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
 +
 
== See also ==
== See also ==
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]

Revision as of 14:32, 14 October 2008

Structure of Triton X-100
Structure of Triton X-100

Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

Contents

Storage/Stability

from the Pierce instructions manual [1]:

  • store at room temperature protected from bright light
  • susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
  • addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated

hydroxytoluene per 500 moles of detergent to prevent autoxidation

Tips

from the Pierce instructions manual [2]:

  • The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm

has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100

  • When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this

form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.

See also

External links

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