Triton X-100: Difference between revisions
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'''Triton''' (full name Triton X-100) is a common detergent used for solubilisation, permeabilisation, and lysis. | [[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]] | ||
'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis. | |||
== Storage/Stability == | |||
* recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf] | |||
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]: | |||
* store at room temperature protected from bright light | |||
* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. | |||
* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated | |||
hydroxytoluene per 500 moles of detergent to prevent autoxidation | |||
== Tips == | |||
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]: | |||
* The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm | |||
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100 | |||
* When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this | |||
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case. | |||
== See also == | |||
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]] | |||
* .. in antibody washing/blocking - [[Silver:Immunofluorescence]] | |||
* .. as part of miniprep - [[Miniprep/Qiagen kit]] | |||
* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]] | |||
* [[Critical micelle concentration (CMC)]] | |||
== External links == | |||
* [http://en.wikipedia.org/wiki/Triton_X-100 Triton at the Wikipedia] | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Detergents]] | [[Category:Detergents]] |
Revision as of 13:04, 29 July 2009
Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
Storage/Stability
- recommended shelf life: 2 years [1]
from the Pierce instructions manual [2]:
- store at room temperature protected from bright light
- susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
- addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
hydroxytoluene per 500 moles of detergent to prevent autoxidation
Tips
from the Pierce instructions manual [3]:
- The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
- When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
See also
- Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
- .. in antibody washing/blocking - Silver:Immunofluorescence
- .. as part of miniprep - Miniprep/Qiagen kit
- .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western
- Critical micelle concentration (CMC)